Abstract
In this study, we synthesized a biocatalyst consisting of glucose oxidase (GOx), polyethyleneimine (PEI) and carbon nanotube (CNT) with addition of glutaraldehyde (GA)(GA/[GOx/PEI/CNT])for fabrication of glucose sensor. Main bonding of the GA/[GOx/PEI/CNT] catalyst was formed by crosslinking of functional end groups between GOx/PEI and GA. Catalytic activity of GA/[GOx/PEI/CNT] was quantified by UV-Vis and electrochemical measurements. As a result of that, high immobilization ratio of 199% than other catalyst (with only physical adsorption) and large sensitivity value of $13.4{\mu}A/cm^2/mM$ was gained. With estimation of the biosensor stability, it was found that the GA/[GOx/PEI/CNT] kept about 88% of its initial activity even after three weeks. It shows GA minimized the loss of GOx and improved sensing ability and stability compared with that using other biocatalysts.
| Translated title of the contribution | Performance Improvement of Glucose Sensor Adopting Enzymatic Catalyst bonded by Glutaraldehyde |
|---|---|
| Original language | Korean |
| Pages (from-to) | 378-385 |
| Number of pages | 8 |
| Journal | 한국수소및신에너지학회논문집 |
| Volume | 27 |
| Issue number | 4 |
| DOIs | |
| State | Published - Aug 2016 |