Abstract
In this paper, we present details on fabrication of single-cell electroporation microdevice, practical experiments of single-cell electroporation with our fabricated microdevice. Also, the continuous electroporation for the continuous flow of cells is used for high-throughput electroporation. The delivery efficiency and cell viability tests are provided and the successful GFP transfection into cells is also evaluated with a fluorescent microscope after electroporation. This device enables to reduce the size of samples and thus the use of small amount of reagents. Also, it makes it possible to permit to avoid cell discrimination (transfected cells versus non-transfected cells)encountered when traditional bulk electroporation is held.
| Translated title of the contribution | Single-cell Electroporation and Gene Transfection using MEMS-based Microdevice with Cantilever-type Microelectrode |
|---|---|
| Original language | Korean |
| Pages (from-to) | 85-91 |
| Number of pages | 7 |
| Journal | 한국정밀공학회지 |
| Volume | 27 |
| Issue number | 5 |
| State | Published - May 2010 |