온도감응성 입자가 포함된 셀룰로오스 하이드로젤의 생체적합성 평가

Carboxymethyl cellulose (CMC) hydrogel has been synthesized in this study by chemically cross-linking of its hydroxyl groups with 1,4-butanediol diglycidyl ether (BDDE), and then converted into a CMC hydrogel by incorporating temperature-responsive (TR) particles with freeze-drying methods. The CMC hydrogel with TR particles was evaluated with physicochemical and biological methods such as color changes over time and temperatures and by in vitro culture of fibroblast cells under the surface of the CMC hydrogels with TR particles, respectively. The CMC hydrogel with TR particles were changed in its colors over temperatures, where its colors depended on the concentrations of TR particles such as 0.1, 0.2 and 0.3%. The results of in vitro assays of the CMC hydrogel with TR particles with bromodeoxy uridune, 3-(4,5-dimethythiazol-2-yl)-2, 5-diphenyl tetrazolium bromide, and neutral red showed excellent cell compatibility compared to those of latex, which was employed as a negative control. Some cells seeded in vitro on the CMC hydrogel were however dead at day 7, even though no significant damages were observed by the extracts of the CMC hydrogel with TR particles. These results indicated that the CMC hydrogel with TR particles should be employed within limited times for its applications in the human contacting areas.