Abstract
The urease is covalently immobilized on the surface of the magnetic particles to catalyze the conversion of
urea to bicarbonate anion. The conversion was confirmed qualitatively using high-pressure liquid chromatography and
UV/Vis spectrometry, and analyzed quantitatively with cyclic voltammetry. The amount of conversion with respect to
time was measured and analyzed by the reaction rate equation to calculate a reaction rate constant of 0.0474 min-1. In the
1 to 3 cycles, a conversion percentage of over 90% was found, and it was possible to reuse the urease 8 times up to the
percentage of 50%. It was also observed that the stability evaluated for storage for 30 days was maintained. As a result
of this study, it can be seen that the urease covalently immobilized on the scaffold can be used for urea removal for the
purpose of producing ultrapure water.
Key words: Urea, Bicarbonate, Enzyme immobilization, Magnetic particles
urea to bicarbonate anion. The conversion was confirmed qualitatively using high-pressure liquid chromatography and
UV/Vis spectrometry, and analyzed quantitatively with cyclic voltammetry. The amount of conversion with respect to
time was measured and analyzed by the reaction rate equation to calculate a reaction rate constant of 0.0474 min-1. In the
1 to 3 cycles, a conversion percentage of over 90% was found, and it was possible to reuse the urease 8 times up to the
percentage of 50%. It was also observed that the stability evaluated for storage for 30 days was maintained. As a result
of this study, it can be seen that the urease covalently immobilized on the scaffold can be used for urea removal for the
purpose of producing ultrapure water.
Key words: Urea, Bicarbonate, Enzyme immobilization, Magnetic particles
| Translated title of the contribution | Urea Conversion via Enzymes Immobilized on Magnetic Microparticles |
|---|---|
| Original language | Korean |
| Pages (from-to) | 407-411 |
| Number of pages | 5 |
| Journal | Korean Chemical Engineering Research(HWAHAK KONGHAK) |
| Volume | 61 |
| Issue number | 3 |
| DOIs | |
| State | Published - Aug 2023 |