항균펩타이드인 Pexiganan (MSI-78) 과 모델 이중 지질막 간의 상호 작용으로생성되는 지질막 파괴에서 Resveratrol 분자의 영향

This study has investigated the effect of Resveratrol(3,5,4'-trihydroxy-trans-stilbene) on membrane fragmentation of model lipid bilayer membranes caused by the antimicrobial peptide, Pexiganan (MSI-78). Large unilamellar vesicles (LUVs) of 7:3 POPC/POPG has been used for the model cellular membrane in the study of interaction between biomolecules and lipid molecules. Recently, this model was used to investigate lipid composition-dependent membrane fragmentation and pore-forming mechanism of membrane disruption by Pexigan (MSI-78). Results showed that the peptide induces micelle-like fragments evidenced by the 31P NMR signal at 0 ppm compared to the typical powder pattern of unperturbed lipid bilayers. In the present study, Resveratrol molecules were added to the LUVs before the peptide was applied to them. 31P spectra were collected for each sample and monitored any changes in the 31p NMR spectra. Our observations show that resveratrol inhibits MSI-78 interaction to POPC/POPG lipid bilayer and protect the lipid membrane evidenced by the disappearance of the isotropic 31P NMR intensity near 0.0 ppm. We suggest that resveratrol binds to the surface of the POPC/POPG bilayers and alters the properties of the lipid membrane.