A novel portable colorimetric probe for fast and selective detection of hypochlorite and its applications

Eunha Han; Yun Seo Lee; Soyeon Kim; Cheal Kim

doi:10.1111/cote.70008

A novel portable colorimetric probe for fast and selective detection of hypochlorite and its applications

Eunha Han, Yun Seo Lee, Soyeon Kim, Cheal Kim

Dept. of Fine Chemistry

Seoul National University of Science and Technology (SNUST)

Research output: Contribution to journal › Article › peer-review

Abstract

A novel portable colorimetric probe TNB (N-(5-(tert-butyl)-1H-pyrazol-3-yl)-7-nitrobenzo[c][1,2,5]oxadiazol-4-amine) was developed to detect hypochlorite (ClO⁻) quickly and simply. Noticeable colour changing from orange to colourless was evident in 1 s, upon the addition of ClO⁻ into TNB. The detection limit of TNB for ClO⁻ was 7.00 μM. TNB could reliably quantify ClO⁻ in real water samples and a commercial bleach. The portable colorimetric probe TNB could obviously discern ClO⁻ in varied media using paper kits. Practically, chemosensor TNB was able to quantify the amounts of ClO⁻ with a smartphone application in environmental samples. The detection mechanism of ClO⁻ was proposed to be hydrolytic cleavage reaction through electrospray ionisation-mass spectrometry (ESI-MS), proton nuclear magnetic resonance (¹H-NMR) analysis, and density functional theory (DFT) calculations.

Original language	English
Journal	Coloration Technology
DOIs	https://doi.org/10.1111/cote.70008
State	Accepted/In press - 2025

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title = "A novel portable colorimetric probe for fast and selective detection of hypochlorite and its applications",

abstract = "A novel portable colorimetric probe TNB (N-(5-(tert-butyl)-1H-pyrazol-3-yl)-7-nitrobenzo[c][1,2,5]oxadiazol-4-amine) was developed to detect hypochlorite (ClO−) quickly and simply. Noticeable colour changing from orange to colourless was evident in 1 s, upon the addition of ClO− into TNB. The detection limit of TNB for ClO− was 7.00 μM. TNB could reliably quantify ClO− in real water samples and a commercial bleach. The portable colorimetric probe TNB could obviously discern ClO− in varied media using paper kits. Practically, chemosensor TNB was able to quantify the amounts of ClO− with a smartphone application in environmental samples. The detection mechanism of ClO− was proposed to be hydrolytic cleavage reaction through electrospray ionisation-mass spectrometry (ESI-MS), proton nuclear magnetic resonance (1H-NMR) analysis, and density functional theory (DFT) calculations.",

author = "Eunha Han and Lee, \{Yun Seo\} and Soyeon Kim and Cheal Kim",

note = "Publisher Copyright: {\textcopyright} 2025 Society of Dyers and Colourists.",

year = "2025",

doi = "10.1111/cote.70008",

language = "English",

journal = "Coloration Technology",

issn = "1472-3581",

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TY - JOUR

T1 - A novel portable colorimetric probe for fast and selective detection of hypochlorite and its applications

AU - Han, Eunha

AU - Lee, Yun Seo

AU - Kim, Soyeon

AU - Kim, Cheal

PY - 2025

Y1 - 2025

N2 - A novel portable colorimetric probe TNB (N-(5-(tert-butyl)-1H-pyrazol-3-yl)-7-nitrobenzo[c][1,2,5]oxadiazol-4-amine) was developed to detect hypochlorite (ClO−) quickly and simply. Noticeable colour changing from orange to colourless was evident in 1 s, upon the addition of ClO− into TNB. The detection limit of TNB for ClO− was 7.00 μM. TNB could reliably quantify ClO− in real water samples and a commercial bleach. The portable colorimetric probe TNB could obviously discern ClO− in varied media using paper kits. Practically, chemosensor TNB was able to quantify the amounts of ClO− with a smartphone application in environmental samples. The detection mechanism of ClO− was proposed to be hydrolytic cleavage reaction through electrospray ionisation-mass spectrometry (ESI-MS), proton nuclear magnetic resonance (1H-NMR) analysis, and density functional theory (DFT) calculations.

AB - A novel portable colorimetric probe TNB (N-(5-(tert-butyl)-1H-pyrazol-3-yl)-7-nitrobenzo[c][1,2,5]oxadiazol-4-amine) was developed to detect hypochlorite (ClO−) quickly and simply. Noticeable colour changing from orange to colourless was evident in 1 s, upon the addition of ClO− into TNB. The detection limit of TNB for ClO− was 7.00 μM. TNB could reliably quantify ClO− in real water samples and a commercial bleach. The portable colorimetric probe TNB could obviously discern ClO− in varied media using paper kits. Practically, chemosensor TNB was able to quantify the amounts of ClO− with a smartphone application in environmental samples. The detection mechanism of ClO− was proposed to be hydrolytic cleavage reaction through electrospray ionisation-mass spectrometry (ESI-MS), proton nuclear magnetic resonance (1H-NMR) analysis, and density functional theory (DFT) calculations.

UR - https://www.scopus.com/pages/publications/105009090164

U2 - 10.1111/cote.70008

DO - 10.1111/cote.70008

M3 - Article

AN - SCOPUS:105009090164

SN - 1472-3581

JO - Coloration Technology

JF - Coloration Technology

ER -

A novel portable colorimetric probe for fast and selective detection of hypochlorite and its applications

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