A novel thiophene-based fluorescent chemosensor for the detection of Zn2+ and Cn−: Imaging applications in live cells and Zebrafish

Min Seon Kim; Dongju Yun; Ju Byeong Chae; Haeri So; Hyojin Lee; Ki Tae Kim; Mingeun Kim; Mi Hee Lim; Cheal Kim

doi:10.3390/s19245458

A novel thiophene-based fluorescent chemosensor for the detection of Zn²⁺ and Cn⁻: Imaging applications in live cells and Zebrafish

Min Seon Kim, Dongju Yun, Ju Byeong Chae, Haeri So, Hyojin Lee, Ki Tae Kim, Mingeun Kim, Mi Hee Lim, Cheal Kim

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

A novel fluorescent turn-on chemosensor DHADC ((E)-3-((4-(diethylamino)-2-hydroxybenzylidene)amino)-2,3-dihydrothiophene-2-carboxamide) has been developed and used to detect Zn²⁺ and CN⁻. Compound DHADC displayed a notable fluorescence increase with Zn²⁺. The limit of detection (2.55 ± 0.05 µM) for zinc ion was far below the standard (76 µM) of the WHO (World Health Organization). In particular, compound DHADC could be applied to determine Zn²⁺ in real samples, and to image Zn²⁺ in both HeLa cells and zebrafish. Additionally, DHADC could detect CN⁻ through a fluorescence enhancement with little inhibition with the existence of other types of anions. The detection processes of compound DHADC for Zn²⁺ and CN⁻ were demonstrated with various analytical methods like Job plots,¹H NMR titrations, and ESI-Mass analyses.

Original language	English
Article number	5458
Journal	Sensors
Volume	19
Issue number	24
DOIs	https://doi.org/10.3390/s19245458
State	Published - 2 Dec 2019

Keywords

Cyanide
Fluorescence chemosensor
Living cell
Zebrafish
Zinc

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title = "A novel thiophene-based fluorescent chemosensor for the detection of Zn2+ and Cn−: Imaging applications in live cells and Zebrafish",

abstract = "A novel fluorescent turn-on chemosensor DHADC ((E)-3-((4-(diethylamino)-2-hydroxybenzylidene)amino)-2,3-dihydrothiophene-2-carboxamide) has been developed and used to detect Zn2+ and CN−. Compound DHADC displayed a notable fluorescence increase with Zn2+. The limit of detection (2.55 ± 0.05 µM) for zinc ion was far below the standard (76 µM) of the WHO (World Health Organization). In particular, compound DHADC could be applied to determine Zn2+ in real samples, and to image Zn2+ in both HeLa cells and zebrafish. Additionally, DHADC could detect CN− through a fluorescence enhancement with little inhibition with the existence of other types of anions. The detection processes of compound DHADC for Zn2+ and CN− were demonstrated with various analytical methods like Job plots,1H NMR titrations, and ESI-Mass analyses.",

keywords = "Cyanide, Fluorescence chemosensor, Living cell, Zebrafish, Zinc",

author = "Kim, \{Min Seon\} and Dongju Yun and Chae, \{Ju Byeong\} and Haeri So and Hyojin Lee and Kim, \{Ki Tae\} and Mingeun Kim and Lim, \{Mi Hee\} and Cheal Kim",

note = "Publisher Copyright: {\textcopyright} 2019 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2019",

month = dec,

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doi = "10.3390/s19245458",

language = "English",

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T2 - Imaging applications in live cells and Zebrafish

AU - Kim, Min Seon

AU - Yun, Dongju

AU - Chae, Ju Byeong

AU - So, Haeri

AU - Lee, Hyojin

AU - Kim, Ki Tae

AU - Kim, Mingeun

AU - Lim, Mi Hee

AU - Kim, Cheal

PY - 2019/12/2

Y1 - 2019/12/2

N2 - A novel fluorescent turn-on chemosensor DHADC ((E)-3-((4-(diethylamino)-2-hydroxybenzylidene)amino)-2,3-dihydrothiophene-2-carboxamide) has been developed and used to detect Zn2+ and CN−. Compound DHADC displayed a notable fluorescence increase with Zn2+. The limit of detection (2.55 ± 0.05 µM) for zinc ion was far below the standard (76 µM) of the WHO (World Health Organization). In particular, compound DHADC could be applied to determine Zn2+ in real samples, and to image Zn2+ in both HeLa cells and zebrafish. Additionally, DHADC could detect CN− through a fluorescence enhancement with little inhibition with the existence of other types of anions. The detection processes of compound DHADC for Zn2+ and CN− were demonstrated with various analytical methods like Job plots,1H NMR titrations, and ESI-Mass analyses.

AB - A novel fluorescent turn-on chemosensor DHADC ((E)-3-((4-(diethylamino)-2-hydroxybenzylidene)amino)-2,3-dihydrothiophene-2-carboxamide) has been developed and used to detect Zn2+ and CN−. Compound DHADC displayed a notable fluorescence increase with Zn2+. The limit of detection (2.55 ± 0.05 µM) for zinc ion was far below the standard (76 µM) of the WHO (World Health Organization). In particular, compound DHADC could be applied to determine Zn2+ in real samples, and to image Zn2+ in both HeLa cells and zebrafish. Additionally, DHADC could detect CN− through a fluorescence enhancement with little inhibition with the existence of other types of anions. The detection processes of compound DHADC for Zn2+ and CN− were demonstrated with various analytical methods like Job plots,1H NMR titrations, and ESI-Mass analyses.

KW - Cyanide

KW - Fluorescence chemosensor

KW - Living cell

KW - Zebrafish

KW - Zinc

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A novel thiophene-based fluorescent chemosensor for the detection of Zn²⁺ and Cn⁻: Imaging applications in live cells and Zebrafish

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Keywords

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