A one-step method for covalent bond immobilization of biomolecules on silica operated in aqueous solution

Yong Kyun Sim; Heetae Jung; Su Hyun Kim; Jung Woo Park; Woo Jin Park; Chul Ho Jun

doi:10.1039/c8sc02565g

A one-step method for covalent bond immobilization of biomolecules on silica operated in aqueous solution

Yong Kyun Sim, Heetae Jung, Su Hyun Kim, Jung Woo Park, Woo Jin Park, Chul Ho Jun

Dept. of Fine Chemistry

Yonsei University

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A simple, one-step method for covalent bond immobilization of biomolecules on silica operated in water is described. In the approach, an NHS-ester linked methallylsilane is utilized as a bifunctional linker to couple the biomolecule to the silica surface. Weak organic acid such as acetic acid activates the silica surface enough to react with bifunctional linker without destroying activity of biomolecule.

Original language	English
Pages (from-to)	7981-7985
Number of pages	5
Journal	Chemical Science
Volume	9
Issue number	41
DOIs	https://doi.org/10.1039/c8sc02565g
State	Published - 2018

Access to Document

10.1039/c8sc02565g

Cite this

@article{f43dc1857ce44d6da5a414290589eb8f,

title = "A one-step method for covalent bond immobilization of biomolecules on silica operated in aqueous solution",

abstract = "A simple, one-step method for covalent bond immobilization of biomolecules on silica operated in water is described. In the approach, an NHS-ester linked methallylsilane is utilized as a bifunctional linker to couple the biomolecule to the silica surface. Weak organic acid such as acetic acid activates the silica surface enough to react with bifunctional linker without destroying activity of biomolecule.",

author = "Sim, \{Yong Kyun\} and Heetae Jung and Kim, \{Su Hyun\} and Park, \{Jung Woo\} and Park, \{Woo Jin\} and Jun, \{Chul Ho\}",

note = "Publisher Copyright: {\textcopyright} 2018 The Royal Society of Chemistry.",

year = "2018",

doi = "10.1039/c8sc02565g",

language = "English",

volume = "9",

pages = "7981--7985",

journal = "Chemical Science",

issn = "2041-6520",

number = "41",

}

TY - JOUR

T1 - A one-step method for covalent bond immobilization of biomolecules on silica operated in aqueous solution

AU - Sim, Yong Kyun

AU - Jung, Heetae

AU - Kim, Su Hyun

AU - Park, Jung Woo

AU - Park, Woo Jin

AU - Jun, Chul Ho

PY - 2018

Y1 - 2018

N2 - A simple, one-step method for covalent bond immobilization of biomolecules on silica operated in water is described. In the approach, an NHS-ester linked methallylsilane is utilized as a bifunctional linker to couple the biomolecule to the silica surface. Weak organic acid such as acetic acid activates the silica surface enough to react with bifunctional linker without destroying activity of biomolecule.

AB - A simple, one-step method for covalent bond immobilization of biomolecules on silica operated in water is described. In the approach, an NHS-ester linked methallylsilane is utilized as a bifunctional linker to couple the biomolecule to the silica surface. Weak organic acid such as acetic acid activates the silica surface enough to react with bifunctional linker without destroying activity of biomolecule.

UR - http://www.scopus.com/inward/record.url?scp=85055634500&partnerID=8YFLogxK

U2 - 10.1039/c8sc02565g

DO - 10.1039/c8sc02565g

M3 - Article

AN - SCOPUS:85055634500

SN - 2041-6520

VL - 9

SP - 7981

EP - 7985

JO - Chemical Science

JF - Chemical Science

IS - 41

ER -

A one-step method for covalent bond immobilization of biomolecules on silica operated in aqueous solution

Abstract

Access to Document

Other files and links

Fingerprint

Cite this