A selective fluorescence sensor for hypochlorite used for the detection of hypochlorite in zebrafish

Pyeong An Kim; Donghwan Choe; Haeri So; Soyoung Park; Boeon Suh; Soomin Jeong; Ki Tae Kim; Cheal Kim; Roger G. Harrison

doi:10.1016/j.saa.2021.120059

A selective fluorescence sensor for hypochlorite used for the detection of hypochlorite in zebrafish

Pyeong An Kim, Donghwan Choe, Haeri So, Soyoung Park, Boeon Suh, Soomin Jeong, Ki Tae Kim, Cheal Kim, Roger G. Harrison

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Hypochlorite is used as a water disinfectant and it is also produced by biological organisms. Its detection and quantification is important and could lead to its mechanism of reactivity in cells. We have synthesized a new fluorescence sensor for hypochlorite based on bithiophene and furan-carbohydrazide. The sensor shows increased fluorescence as a function of hypochlorite and is selective for hypochlorite. Fluorescence enhancement due to hypochlorite is observed when the sensor is used in aqueous solutions at neutral pH values. Using the sensor, the detection limit for hypochlorite is 4.2 µM, making the sensor practical to determine hypochlorite in water. Applying the sensor to aide in the detection of hypochlorite in zebrafish, showed localization of ClO⁻/HClO in the air bladders and eyes of zebrafish.

Original language	English
Article number	120059
Journal	Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume	261
DOIs	https://doi.org/10.1016/j.saa.2021.120059
State	Published - 15 Nov 2021

Keywords

Fluorescent probe
Hypochlorite sensor
Imine oxidation
Zebrafish

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10.1016/j.saa.2021.120059

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title = "A selective fluorescence sensor for hypochlorite used for the detection of hypochlorite in zebrafish",

abstract = "Hypochlorite is used as a water disinfectant and it is also produced by biological organisms. Its detection and quantification is important and could lead to its mechanism of reactivity in cells. We have synthesized a new fluorescence sensor for hypochlorite based on bithiophene and furan-carbohydrazide. The sensor shows increased fluorescence as a function of hypochlorite and is selective for hypochlorite. Fluorescence enhancement due to hypochlorite is observed when the sensor is used in aqueous solutions at neutral pH values. Using the sensor, the detection limit for hypochlorite is 4.2 µM, making the sensor practical to determine hypochlorite in water. Applying the sensor to aide in the detection of hypochlorite in zebrafish, showed localization of ClO−/HClO in the air bladders and eyes of zebrafish.",

keywords = "Fluorescent probe, Hypochlorite sensor, Imine oxidation, Zebrafish",

author = "Kim, \{Pyeong An\} and Donghwan Choe and Haeri So and Soyoung Park and Boeon Suh and Soomin Jeong and Kim, \{Ki Tae\} and Cheal Kim and Harrison, \{Roger G.\}",

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doi = "10.1016/j.saa.2021.120059",

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T1 - A selective fluorescence sensor for hypochlorite used for the detection of hypochlorite in zebrafish

AU - Kim, Pyeong An

AU - Choe, Donghwan

AU - So, Haeri

AU - Park, Soyoung

AU - Suh, Boeon

AU - Jeong, Soomin

AU - Kim, Ki Tae

AU - Kim, Cheal

AU - Harrison, Roger G.

PY - 2021/11/15

Y1 - 2021/11/15

N2 - Hypochlorite is used as a water disinfectant and it is also produced by biological organisms. Its detection and quantification is important and could lead to its mechanism of reactivity in cells. We have synthesized a new fluorescence sensor for hypochlorite based on bithiophene and furan-carbohydrazide. The sensor shows increased fluorescence as a function of hypochlorite and is selective for hypochlorite. Fluorescence enhancement due to hypochlorite is observed when the sensor is used in aqueous solutions at neutral pH values. Using the sensor, the detection limit for hypochlorite is 4.2 µM, making the sensor practical to determine hypochlorite in water. Applying the sensor to aide in the detection of hypochlorite in zebrafish, showed localization of ClO−/HClO in the air bladders and eyes of zebrafish.

AB - Hypochlorite is used as a water disinfectant and it is also produced by biological organisms. Its detection and quantification is important and could lead to its mechanism of reactivity in cells. We have synthesized a new fluorescence sensor for hypochlorite based on bithiophene and furan-carbohydrazide. The sensor shows increased fluorescence as a function of hypochlorite and is selective for hypochlorite. Fluorescence enhancement due to hypochlorite is observed when the sensor is used in aqueous solutions at neutral pH values. Using the sensor, the detection limit for hypochlorite is 4.2 µM, making the sensor practical to determine hypochlorite in water. Applying the sensor to aide in the detection of hypochlorite in zebrafish, showed localization of ClO−/HClO in the air bladders and eyes of zebrafish.

KW - Fluorescent probe

KW - Hypochlorite sensor

KW - Imine oxidation

KW - Zebrafish

UR - https://www.scopus.com/pages/publications/85108102581

U2 - 10.1016/j.saa.2021.120059

DO - 10.1016/j.saa.2021.120059

M3 - Article

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AN - SCOPUS:85108102581

SN - 1386-1425

VL - 261

JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

M1 - 120059

ER -

A selective fluorescence sensor for hypochlorite used for the detection of hypochlorite in zebrafish

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Keywords

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