TY - JOUR
T1 - A Self-Powered Dual ROS Sensor Adopting Biofuel Cell Platform for Real-Time and Selective Monitoring of Oxygen and Hydrogen Peroxide
AU - Lee, Joonyoung
AU - Kwon, Yongchai
N1 - Publisher Copyright:
© The Author(s), under exclusive licence to Korean Institute of Chemical Engineers 2025.
PY - 2025/9
Y1 - 2025/9
N2 - Reactive oxygen species (ROS) play a crucial role in various biological processes, and their accurate detection is essential for biomedical applications. Although various types of ROS sensors are explored, there are demands for sensors that can be applied to wearable and implantable devices to measure the concentration of ROS in the human body. In this study, a self-powered ROS sensor is explored based on enzymatic biofuel cell (EBFC) to selectively detect oxygen (O2) and hydrogen peroxide (H2O2). Furthermore, this ROS sensor utilizes buckypaper and polydimethylsiloxane (BP@PDMS)-based electrode. For anode, glucose dehydrogenase is immobilized on BP@PDMS, while as cathode, both bilirubin oxidase (BOD) and horseradish peroxidase (HRP) are immobilized on BP@PDMS, and the two cathodes detect O2 and H2O2, respectively. They show good sensitivity for each O2 and H2O2 fuel, while the sensitivity is quantified by measuring their reduction current density. Furthermore, polarization curves of full cell prepared with one anode and two cathodes show maximum power density of 129 µW/cm2 at 0.4 V for O2 and 440 µW/cm2 at 0.5 V for H2O2, and this proves desirable step reaction occurs within the given concentration range of fuels, which are 25–100 cc/min (O2) and 1–3 mM (H2O2). Furthermore, the flexible design of self-powered ROS sensor explored in this study highlights its possibility for integration into wearable and implantable devices, while this study proves that ROS sensor adopting EBFC platform can show high sensitivity and selectivity, and excellent adaptability for associated applications.
AB - Reactive oxygen species (ROS) play a crucial role in various biological processes, and their accurate detection is essential for biomedical applications. Although various types of ROS sensors are explored, there are demands for sensors that can be applied to wearable and implantable devices to measure the concentration of ROS in the human body. In this study, a self-powered ROS sensor is explored based on enzymatic biofuel cell (EBFC) to selectively detect oxygen (O2) and hydrogen peroxide (H2O2). Furthermore, this ROS sensor utilizes buckypaper and polydimethylsiloxane (BP@PDMS)-based electrode. For anode, glucose dehydrogenase is immobilized on BP@PDMS, while as cathode, both bilirubin oxidase (BOD) and horseradish peroxidase (HRP) are immobilized on BP@PDMS, and the two cathodes detect O2 and H2O2, respectively. They show good sensitivity for each O2 and H2O2 fuel, while the sensitivity is quantified by measuring their reduction current density. Furthermore, polarization curves of full cell prepared with one anode and two cathodes show maximum power density of 129 µW/cm2 at 0.4 V for O2 and 440 µW/cm2 at 0.5 V for H2O2, and this proves desirable step reaction occurs within the given concentration range of fuels, which are 25–100 cc/min (O2) and 1–3 mM (H2O2). Furthermore, the flexible design of self-powered ROS sensor explored in this study highlights its possibility for integration into wearable and implantable devices, while this study proves that ROS sensor adopting EBFC platform can show high sensitivity and selectivity, and excellent adaptability for associated applications.
KW - Bilirubin oxidase
KW - Buckypaper
KW - Enzymatic biofuel cell
KW - Hydrogen peroxide
KW - Polydimethylsiloxane
KW - ROS sensor
UR - https://www.scopus.com/pages/publications/105012304707
U2 - 10.1007/s11814-025-00530-0
DO - 10.1007/s11814-025-00530-0
M3 - Article
AN - SCOPUS:105012304707
SN - 0256-1115
VL - 42
SP - 2685
EP - 2691
JO - Korean Journal of Chemical Engineering
JF - Korean Journal of Chemical Engineering
IS - 11
ER -
