A thiourea-based fluorescent chemosensor for bioimaging hypochlorite

Haeri So; Hangyul Lee; Gyu Dong Lee; Mingeun Kim; Mi Hee Lim; Ki Tae Kim; Cheal Kim

doi:10.1016/j.jiec.2020.06.016

A thiourea-based fluorescent chemosensor for bioimaging hypochlorite

Haeri So, Hangyul Lee, Gyu Dong Lee, Mingeun Kim, Mi Hee Lim, Ki Tae Kim, Cheal Kim

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

A thiourea-based fluorescent turn-off chemosensor FHC was developed for recognizing hypochlorite. With the addition of hypochlorite to FHC, FHC displayed a specific desulfurization reaction of the thiourea group followed by the fluorescent quenching response. The fluorescent turn-off response of FHC to hypochlorite was very fast to finish within a few seconds. Detection limit of hypochlorite was determined to be 0.43 μM. FHC can be applied for the bioimaging of hypochlorite in both HeLa cells and zebrafish. Response process of FHC to hypochlorite, via the desulfurization of the thiourea moiety, was demonstrated, based on ¹H NMR titrations, ESI-mass, fluorescent and UV–vis titrations and DFT calculations.

Original language	English
Pages (from-to)	436-441
Number of pages	6
Journal	Journal of Industrial and Engineering Chemistry
Volume	89
DOIs	https://doi.org/10.1016/j.jiec.2020.06.016
State	Published - 25 Sep 2020

Keywords

Cells
Fluorescence Chemosensor
Hypochlorite
Thiourea
Zebrafish

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10.1016/j.jiec.2020.06.016

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title = "A thiourea-based fluorescent chemosensor for bioimaging hypochlorite",

abstract = "A thiourea-based fluorescent turn-off chemosensor FHC was developed for recognizing hypochlorite. With the addition of hypochlorite to FHC, FHC displayed a specific desulfurization reaction of the thiourea group followed by the fluorescent quenching response. The fluorescent turn-off response of FHC to hypochlorite was very fast to finish within a few seconds. Detection limit of hypochlorite was determined to be 0.43 μM. FHC can be applied for the bioimaging of hypochlorite in both HeLa cells and zebrafish. Response process of FHC to hypochlorite, via the desulfurization of the thiourea moiety, was demonstrated, based on 1H NMR titrations, ESI-mass, fluorescent and UV–vis titrations and DFT calculations.",

keywords = "Cells, Fluorescence Chemosensor, Hypochlorite, Thiourea, Zebrafish",

author = "Haeri So and Hangyul Lee and Lee, \{Gyu Dong\} and Mingeun Kim and Lim, \{Mi Hee\} and Kim, \{Ki Tae\} and Cheal Kim",

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year = "2020",

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doi = "10.1016/j.jiec.2020.06.016",

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T1 - A thiourea-based fluorescent chemosensor for bioimaging hypochlorite

AU - So, Haeri

AU - Lee, Hangyul

AU - Lee, Gyu Dong

AU - Kim, Mingeun

AU - Lim, Mi Hee

AU - Kim, Ki Tae

AU - Kim, Cheal

PY - 2020/9/25

Y1 - 2020/9/25

N2 - A thiourea-based fluorescent turn-off chemosensor FHC was developed for recognizing hypochlorite. With the addition of hypochlorite to FHC, FHC displayed a specific desulfurization reaction of the thiourea group followed by the fluorescent quenching response. The fluorescent turn-off response of FHC to hypochlorite was very fast to finish within a few seconds. Detection limit of hypochlorite was determined to be 0.43 μM. FHC can be applied for the bioimaging of hypochlorite in both HeLa cells and zebrafish. Response process of FHC to hypochlorite, via the desulfurization of the thiourea moiety, was demonstrated, based on 1H NMR titrations, ESI-mass, fluorescent and UV–vis titrations and DFT calculations.

AB - A thiourea-based fluorescent turn-off chemosensor FHC was developed for recognizing hypochlorite. With the addition of hypochlorite to FHC, FHC displayed a specific desulfurization reaction of the thiourea group followed by the fluorescent quenching response. The fluorescent turn-off response of FHC to hypochlorite was very fast to finish within a few seconds. Detection limit of hypochlorite was determined to be 0.43 μM. FHC can be applied for the bioimaging of hypochlorite in both HeLa cells and zebrafish. Response process of FHC to hypochlorite, via the desulfurization of the thiourea moiety, was demonstrated, based on 1H NMR titrations, ESI-mass, fluorescent and UV–vis titrations and DFT calculations.

KW - Cells

KW - Fluorescence Chemosensor

KW - Hypochlorite

KW - Thiourea

KW - Zebrafish

UR - https://www.scopus.com/pages/publications/85087381267

U2 - 10.1016/j.jiec.2020.06.016

DO - 10.1016/j.jiec.2020.06.016

M3 - Article

AN - SCOPUS:85087381267

SN - 1226-086X

VL - 89

SP - 436

EP - 441

JO - Journal of Industrial and Engineering Chemistry

JF - Journal of Industrial and Engineering Chemistry

ER -

A thiourea-based fluorescent chemosensor for bioimaging hypochlorite

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Keywords

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