TY - JOUR
T1 - Anti-inflammatory effects of ethanolic mulberry extract on the murine macrophage cell line, RAW 264.7
AU - Kim, Yunyoung
AU - Yang, Yoon Kyoung
AU - Kim, Dongmin
AU - Kim, Ji Yeon
PY - 2017/6
Y1 - 2017/6
N2 - The aim of this study was to compare the anti-inflammatory effects of ethanol extracts of root peel and spear of mulberry (RME and SME, respectively) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Both extracts significantly inhibited the production of reactive oxygen species (ROS), nitric oxide (NO), and interleukin-6 (IL-6). However, prostaglandin E2 (PGE2) levels decreased in LPS-stimulated RAW 264.7 cells treated with SME. Additionally, the extracts reduced inducible NO synthase (iNOS) expression and cyclooxygenase-2 (COX-2) in mRNA levels. Although ROS production was lower in the RME-treated cells than in the SME-treated cells, the levels of other inflammatory parameters, including IL-6 and PGE2, and mRNA levels of iNOS and COX-2 reduced more in the SME-treated cells. These results indicate that SME showed higher anti-inflammatory activities than RME. Therefore, SME can be used as a functional food ingredient to enhance health.
AB - The aim of this study was to compare the anti-inflammatory effects of ethanol extracts of root peel and spear of mulberry (RME and SME, respectively) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Both extracts significantly inhibited the production of reactive oxygen species (ROS), nitric oxide (NO), and interleukin-6 (IL-6). However, prostaglandin E2 (PGE2) levels decreased in LPS-stimulated RAW 264.7 cells treated with SME. Additionally, the extracts reduced inducible NO synthase (iNOS) expression and cyclooxygenase-2 (COX-2) in mRNA levels. Although ROS production was lower in the RME-treated cells than in the SME-treated cells, the levels of other inflammatory parameters, including IL-6 and PGE2, and mRNA levels of iNOS and COX-2 reduced more in the SME-treated cells. These results indicate that SME showed higher anti-inflammatory activities than RME. Therefore, SME can be used as a functional food ingredient to enhance health.
KW - Anti-inflammatory
KW - RAW 264.7 cell
KW - Root peel of Mulberry ethanol extract
KW - Spear of Mulberry ethanol extract
UR - https://www.scopus.com/pages/publications/85040768244
U2 - 10.9721/KJFST.2017.49.3.343
DO - 10.9721/KJFST.2017.49.3.343
M3 - Article
AN - SCOPUS:85040768244
SN - 0367-6293
VL - 49
SP - 343
EP - 348
JO - Korean Journal of Food Science and Technology
JF - Korean Journal of Food Science and Technology
IS - 3
ER -