TY - JOUR
T1 - Antibacterial and antioxidant activities of leaf extracts from juglans sinensis, and its phenolic compositions
AU - Han, Kook Il
AU - Jung, Eui Gil
AU - Patnaik, Bharat Bhusan
AU - Hong, Chang Il
AU - Kim, Young Jun
AU - Jung, Seunghyeon
AU - Han, Man Deuk
PY - 2017/11
Y1 - 2017/11
N2 - The beneficial properties of walnut depend on climate, genotype, and farming practices. We investigated the antibacterial and antioxidant activities of Juglans sinensis Dode cultivars grown in South Korea. Antibacterial activities were assessed using the minimum inhibitory concentration method and antioxidant activities using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assays. Ethyl acetate and chloroform extracts of J. sinensis leaves showed the highest antibacterial activity against Staphylococcus aureus SG511, Klebsiella aerogenes 1522E, and/or Enterobacter cloacae P99. DPPH and ABTS radical scavenging activities were found to be highest in the ethyl acetate extract. The total flavonoid and polyphenolic contents in the extract were 219.27 mg/g quercetin equivalents and 245.11 mg/g of gallic acid equivalents, respectively. Phenolic acids (gallic and vanillic) and flavonoids (catechin, rutin, and juglone) were detected by high performance liquid chromatography. We conclude that J. sinensis cultivars provide accessible sources of natural antibacterials and antioxidants.
AB - The beneficial properties of walnut depend on climate, genotype, and farming practices. We investigated the antibacterial and antioxidant activities of Juglans sinensis Dode cultivars grown in South Korea. Antibacterial activities were assessed using the minimum inhibitory concentration method and antioxidant activities using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assays. Ethyl acetate and chloroform extracts of J. sinensis leaves showed the highest antibacterial activity against Staphylococcus aureus SG511, Klebsiella aerogenes 1522E, and/or Enterobacter cloacae P99. DPPH and ABTS radical scavenging activities were found to be highest in the ethyl acetate extract. The total flavonoid and polyphenolic contents in the extract were 219.27 mg/g quercetin equivalents and 245.11 mg/g of gallic acid equivalents, respectively. Phenolic acids (gallic and vanillic) and flavonoids (catechin, rutin, and juglone) were detected by high performance liquid chromatography. We conclude that J. sinensis cultivars provide accessible sources of natural antibacterials and antioxidants.
KW - Antibacterial activity
KW - Antioxidant activity
KW - Juglans sinensis
KW - Phenolic acids
KW - Reversed-phase high-performance liquid chromatography
UR - http://www.scopus.com/inward/record.url?scp=85035194380&partnerID=8YFLogxK
U2 - 10.1177/1934578x1701201138
DO - 10.1177/1934578x1701201138
M3 - Article
AN - SCOPUS:85035194380
SN - 1934-578X
VL - 12
SP - 1797
EP - 1800
JO - Natural Product Communications
JF - Natural Product Communications
IS - 11
ER -