Biomimetic whitlockite inorganic nanoparticles-mediated in situ remodeling and rapid bone regeneration

Hwan D. Kim; Hae Lin Jang; Hyo Yong Ahn; Hye Kyoung Lee; Jungha Park; Eun seo Lee; Eunjee A. Lee; Yong Hoon Jeong; Do Gyoon Kim; Ki Tae Nam; Nathaniel S. Hwang

doi:10.1016/j.biomaterials.2016.10.009

Biomimetic whitlockite inorganic nanoparticles-mediated in situ remodeling and rapid bone regeneration

Hwan D. Kim
, Hae Lin Jang
, Hyo Yong Ahn
, Hye Kyoung Lee
, Jungha Park
, Eun seo Lee
, Eunjee A. Lee
, Yong Hoon Jeong
, Do Gyoon Kim
, Ki Tae Nam
, Nathaniel S. Hwang

College of Energy and Biotechnology

Research output: Contribution to journal › Article › peer-review

181 Scopus citations

Abstract

Bone remodeling process relies on complex signaling pathway between osteoblasts and osteoclasts and control mechanisms to achieve homeostasis of their growth and differentiation. Despite previous achievements in understanding complicated signaling pathways between cells and bone extracellular matrices during bone remodeling process, a role of local ionic concentration remains to be elucidated. Here, we demonstrate that synthetic whitlockite (WH: Ca₁₈Mg₂(HPO₄)₂(PO₄)₁₂) nanoparticles can recapitulate early-stage of bone regeneration through stimulating osteogenic differentiation, prohibiting osteoclastic activity, and transforming into mechanically enhanced hydroxyapatite (HAP)-neo bone tissues by continuous supply of PO₄³⁻ and Mg²⁺ under physiological conditions. In addition, based on their structural analysis, the dynamic phase transformation from WH into HAP contributed as a key factor for rapid bone regeneration with denser hierarchical neo-bone structure. Our findings suggest a groundbreaking concept of ‘living bone minerals’ that actively communicate with the surrounding system to induce self-healing, while previous notions about bone minerals have been limited to passive products of cellular mineralization.

Original language	English
Pages (from-to)	31-43
Number of pages	13
Journal	Biomaterials
Volume	112
DOIs	https://doi.org/10.1016/j.biomaterials.2016.10.009
State	Published - 1 Jan 2017

Keywords

Bone remodeling process
Bone tissue engineering
Cryogel
Inorganic phosphate
Ion metabolic pathway
Nanoparticles
Whitlockite

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10.1016/j.biomaterials.2016.10.009

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title = "Biomimetic whitlockite inorganic nanoparticles-mediated in situ remodeling and rapid bone regeneration",

abstract = "Bone remodeling process relies on complex signaling pathway between osteoblasts and osteoclasts and control mechanisms to achieve homeostasis of their growth and differentiation. Despite previous achievements in understanding complicated signaling pathways between cells and bone extracellular matrices during bone remodeling process, a role of local ionic concentration remains to be elucidated. Here, we demonstrate that synthetic whitlockite (WH: Ca18Mg2(HPO4)2(PO4)12) nanoparticles can recapitulate early-stage of bone regeneration through stimulating osteogenic differentiation, prohibiting osteoclastic activity, and transforming into mechanically enhanced hydroxyapatite (HAP)-neo bone tissues by continuous supply of PO43− and Mg2+ under physiological conditions. In addition, based on their structural analysis, the dynamic phase transformation from WH into HAP contributed as a key factor for rapid bone regeneration with denser hierarchical neo-bone structure. Our findings suggest a groundbreaking concept of {\textquoteleft}living bone minerals{\textquoteright} that actively communicate with the surrounding system to induce self-healing, while previous notions about bone minerals have been limited to passive products of cellular mineralization.",

keywords = "Bone remodeling process, Bone tissue engineering, Cryogel, Inorganic phosphate, Ion metabolic pathway, Nanoparticles, Whitlockite",

author = "Kim, \{Hwan D.\} and Jang, \{Hae Lin\} and Ahn, \{Hyo Yong\} and Lee, \{Hye Kyoung\} and Jungha Park and Lee, \{Eun seo\} and Lee, \{Eunjee A.\} and Jeong, \{Yong Hoon\} and Kim, \{Do Gyoon\} and Nam, \{Ki Tae\} and Hwang, \{Nathaniel S.\}",

note = "Publisher Copyright: {\textcopyright} 2016",

year = "2017",

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doi = "10.1016/j.biomaterials.2016.10.009",

language = "English",

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AU - Kim, Hwan D.

AU - Jang, Hae Lin

AU - Ahn, Hyo Yong

AU - Lee, Hye Kyoung

AU - Park, Jungha

AU - Lee, Eun seo

AU - Lee, Eunjee A.

AU - Jeong, Yong Hoon

AU - Kim, Do Gyoon

AU - Nam, Ki Tae

AU - Hwang, Nathaniel S.

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Bone remodeling process relies on complex signaling pathway between osteoblasts and osteoclasts and control mechanisms to achieve homeostasis of their growth and differentiation. Despite previous achievements in understanding complicated signaling pathways between cells and bone extracellular matrices during bone remodeling process, a role of local ionic concentration remains to be elucidated. Here, we demonstrate that synthetic whitlockite (WH: Ca18Mg2(HPO4)2(PO4)12) nanoparticles can recapitulate early-stage of bone regeneration through stimulating osteogenic differentiation, prohibiting osteoclastic activity, and transforming into mechanically enhanced hydroxyapatite (HAP)-neo bone tissues by continuous supply of PO43− and Mg2+ under physiological conditions. In addition, based on their structural analysis, the dynamic phase transformation from WH into HAP contributed as a key factor for rapid bone regeneration with denser hierarchical neo-bone structure. Our findings suggest a groundbreaking concept of ‘living bone minerals’ that actively communicate with the surrounding system to induce self-healing, while previous notions about bone minerals have been limited to passive products of cellular mineralization.

AB - Bone remodeling process relies on complex signaling pathway between osteoblasts and osteoclasts and control mechanisms to achieve homeostasis of their growth and differentiation. Despite previous achievements in understanding complicated signaling pathways between cells and bone extracellular matrices during bone remodeling process, a role of local ionic concentration remains to be elucidated. Here, we demonstrate that synthetic whitlockite (WH: Ca18Mg2(HPO4)2(PO4)12) nanoparticles can recapitulate early-stage of bone regeneration through stimulating osteogenic differentiation, prohibiting osteoclastic activity, and transforming into mechanically enhanced hydroxyapatite (HAP)-neo bone tissues by continuous supply of PO43− and Mg2+ under physiological conditions. In addition, based on their structural analysis, the dynamic phase transformation from WH into HAP contributed as a key factor for rapid bone regeneration with denser hierarchical neo-bone structure. Our findings suggest a groundbreaking concept of ‘living bone minerals’ that actively communicate with the surrounding system to induce self-healing, while previous notions about bone minerals have been limited to passive products of cellular mineralization.

KW - Bone remodeling process

KW - Bone tissue engineering

KW - Cryogel

KW - Inorganic phosphate

KW - Ion metabolic pathway

KW - Nanoparticles

KW - Whitlockite

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DO - 10.1016/j.biomaterials.2016.10.009

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SN - 0142-9612

VL - 112

SP - 31

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JO - Biomaterials

JF - Biomaterials

ER -

Biomimetic whitlockite inorganic nanoparticles-mediated in situ remodeling and rapid bone regeneration

Abstract

Keywords

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