Cytoplasmic fraction of Lactococcus lactis ssp. lactis induces apoptosis in SNU-1 stomach adenocarcinoma cells

Young Kim Seo; Won Lee Ki; Yeon Kim Ji; Joo Lee Hyong

doi:10.1002/biof.5520220123

Cytoplasmic fraction of Lactococcus lactis ssp. lactis induces apoptosis in SNU-1 stomach adenocarcinoma cells

Young Kim Seo, Won Lee Ki, Yeon Kim Ji, Joo Lee Hyong

Dept. of Food Science and Technology

Seoul National University

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Lactic acid bacteria are known to have antitumor activity, but the underlying mechanisms remain unclear. Recently we showed that a cytoplasmic fraction - but not peptidoglycan - of Lactococcus lactis ssp. lactis (L.lac CF) had strong antiproliferative activity on SNU-1 human stomach adenocarcinoma cells. The present study investigated whether the antiproliferative activity of L.lac CF on SNU-1 is linked to the induction of apoptosis. Treatment of L.lac CF inhibited the proliferation of SNU-1 cells in a dose- and time-dependent manner. Furthermore, treatment of the cells with 50 μg/ml and 100 μg/ml L.lac CF resulted in DNA fragmentation and chromatin condensation, respectively. The results indicate that the inhibitory effect of L.lac CF on SNU-1 cell growth is mainly attributable to the induction of apoptosis.

Original language	English
Pages (from-to)	119-122
Number of pages	4
Journal	BioFactors
Volume	22
Issue number	1-4
DOIs	https://doi.org/10.1002/biof.5520220123
State	Published - 2005

Keywords

Apoptosis
Cytoplasmic fraction
Lactococcus lactis ssp. lactis
SNU-1 human stomach adenocarcinoma cell line

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/biof.5520220123

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title = "Cytoplasmic fraction of Lactococcus lactis ssp. lactis induces apoptosis in SNU-1 stomach adenocarcinoma cells",

abstract = "Lactic acid bacteria are known to have antitumor activity, but the underlying mechanisms remain unclear. Recently we showed that a cytoplasmic fraction - but not peptidoglycan - of Lactococcus lactis ssp. lactis (L.lac CF) had strong antiproliferative activity on SNU-1 human stomach adenocarcinoma cells. The present study investigated whether the antiproliferative activity of L.lac CF on SNU-1 is linked to the induction of apoptosis. Treatment of L.lac CF inhibited the proliferation of SNU-1 cells in a dose- and time-dependent manner. Furthermore, treatment of the cells with 50 μg/ml and 100 μg/ml L.lac CF resulted in DNA fragmentation and chromatin condensation, respectively. The results indicate that the inhibitory effect of L.lac CF on SNU-1 cell growth is mainly attributable to the induction of apoptosis.",

keywords = "Apoptosis, Cytoplasmic fraction, Lactococcus lactis ssp. lactis, SNU-1 human stomach adenocarcinoma cell line",

author = "Seo, \{Young Kim\} and Ki, \{Won Lee\} and Ji, \{Yeon Kim\} and Hyong, \{Joo Lee\}",

year = "2005",

doi = "10.1002/biof.5520220123",

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volume = "22",

pages = "119--122",

journal = "BioFactors",

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TY - JOUR

T1 - Cytoplasmic fraction of Lactococcus lactis ssp. lactis induces apoptosis in SNU-1 stomach adenocarcinoma cells

AU - Seo, Young Kim

AU - Ki, Won Lee

AU - Ji, Yeon Kim

AU - Hyong, Joo Lee

PY - 2005

Y1 - 2005

N2 - Lactic acid bacteria are known to have antitumor activity, but the underlying mechanisms remain unclear. Recently we showed that a cytoplasmic fraction - but not peptidoglycan - of Lactococcus lactis ssp. lactis (L.lac CF) had strong antiproliferative activity on SNU-1 human stomach adenocarcinoma cells. The present study investigated whether the antiproliferative activity of L.lac CF on SNU-1 is linked to the induction of apoptosis. Treatment of L.lac CF inhibited the proliferation of SNU-1 cells in a dose- and time-dependent manner. Furthermore, treatment of the cells with 50 μg/ml and 100 μg/ml L.lac CF resulted in DNA fragmentation and chromatin condensation, respectively. The results indicate that the inhibitory effect of L.lac CF on SNU-1 cell growth is mainly attributable to the induction of apoptosis.

AB - Lactic acid bacteria are known to have antitumor activity, but the underlying mechanisms remain unclear. Recently we showed that a cytoplasmic fraction - but not peptidoglycan - of Lactococcus lactis ssp. lactis (L.lac CF) had strong antiproliferative activity on SNU-1 human stomach adenocarcinoma cells. The present study investigated whether the antiproliferative activity of L.lac CF on SNU-1 is linked to the induction of apoptosis. Treatment of L.lac CF inhibited the proliferation of SNU-1 cells in a dose- and time-dependent manner. Furthermore, treatment of the cells with 50 μg/ml and 100 μg/ml L.lac CF resulted in DNA fragmentation and chromatin condensation, respectively. The results indicate that the inhibitory effect of L.lac CF on SNU-1 cell growth is mainly attributable to the induction of apoptosis.

KW - Apoptosis

KW - Cytoplasmic fraction

KW - Lactococcus lactis ssp. lactis

KW - SNU-1 human stomach adenocarcinoma cell line

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U2 - 10.1002/biof.5520220123

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AN - SCOPUS:16244386854

SN - 0951-6433

VL - 22

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JO - BioFactors

JF - BioFactors

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ER -

Cytoplasmic fraction of Lactococcus lactis ssp. lactis induces apoptosis in SNU-1 stomach adenocarcinoma cells

Abstract

Keywords

UN SDGs

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