Detection of Bacillus Cereus Using Bioluminescence Assay with Cell Wall-binding Domain Conjugated Magnetic Nanoparticles

Chanyong Park; Minsuk Kong; Ju Hoon Lee; Sangryeol Ryu; Sungsu Park

doi:10.1007/s13206-018-2408-8

Detection of Bacillus Cereus Using Bioluminescence Assay with Cell Wall-binding Domain Conjugated Magnetic Nanoparticles

Chanyong Park, Minsuk Kong, Ju Hoon Lee, Sangryeol Ryu, Sungsu Park

Dept. of Food Science and Technology

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Bacillus cereus can cause blood infections (i.e., sepsis). Its early detection is very important for treating patients. However, an antibody with high binding affinity to B. cereus is not currently available. Bacteriophage cell wall-binding domain (CBD) has strong and specific binding affinity to B. cereus. Here, we report the improvement in the sensitivity of an ATP bioluminescence assay for B. cereus detection using CBD-conjugated magnetic nanoparticles (CBDMNPs). The assay was able to detect as few as 10 colony forming units (CFU) per mL and 10 ³ CFU per mL in buffer and blood. CBD-MNPs did not show any cross-reactivity with other microorganisms. These results demonstrate the feasibility of the ATP assay for the detection of B. cereus.

Original language	English
Pages (from-to)	287-293
Number of pages	7
Journal	Biochip Journal
Volume	12
Issue number	4
DOIs	https://doi.org/10.1007/s13206-018-2408-8
State	Published - 1 Dec 2018

Keywords

ATP bioluminescence assay
Bacillus cereus
Cell wall-binding domain
Enrichment
Magnetic nanoparticles

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10.1007/s13206-018-2408-8

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title = "Detection of Bacillus Cereus Using Bioluminescence Assay with Cell Wall-binding Domain Conjugated Magnetic Nanoparticles",

abstract = " Bacillus cereus can cause blood infections (i.e., sepsis). Its early detection is very important for treating patients. However, an antibody with high binding affinity to B. cereus is not currently available. Bacteriophage cell wall-binding domain (CBD) has strong and specific binding affinity to B. cereus. Here, we report the improvement in the sensitivity of an ATP bioluminescence assay for B. cereus detection using CBD-conjugated magnetic nanoparticles (CBDMNPs). The assay was able to detect as few as 10 colony forming units (CFU) per mL and 10 3 CFU per mL in buffer and blood. CBD-MNPs did not show any cross-reactivity with other microorganisms. These results demonstrate the feasibility of the ATP assay for the detection of B. cereus.",

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author = "Chanyong Park and Minsuk Kong and Lee, \{Ju Hoon\} and Sangryeol Ryu and Sungsu Park",

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AU - Park, Chanyong

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AU - Ryu, Sangryeol

AU - Park, Sungsu

PY - 2018/12/1

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AB - Bacillus cereus can cause blood infections (i.e., sepsis). Its early detection is very important for treating patients. However, an antibody with high binding affinity to B. cereus is not currently available. Bacteriophage cell wall-binding domain (CBD) has strong and specific binding affinity to B. cereus. Here, we report the improvement in the sensitivity of an ATP bioluminescence assay for B. cereus detection using CBD-conjugated magnetic nanoparticles (CBDMNPs). The assay was able to detect as few as 10 colony forming units (CFU) per mL and 10 3 CFU per mL in buffer and blood. CBD-MNPs did not show any cross-reactivity with other microorganisms. These results demonstrate the feasibility of the ATP assay for the detection of B. cereus.

KW - ATP bioluminescence assay

KW - Bacillus cereus

KW - Cell wall-binding domain

KW - Enrichment

KW - Magnetic nanoparticles

UR - https://www.scopus.com/pages/publications/85058800883

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DO - 10.1007/s13206-018-2408-8

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Detection of Bacillus Cereus Using Bioluminescence Assay with Cell Wall-binding Domain Conjugated Magnetic Nanoparticles

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Keywords

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