Effect of aminoglycoside on mechanical properties of zwitterionic phospholipid vesicles

Jin Won Park

doi:10.1016/j.colsurfb.2011.07.046

Effect of aminoglycoside on mechanical properties of zwitterionic phospholipid vesicles

Jin Won Park

Dept. of Chemical and Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The effect of the aminoglycoside (streptomycin) incorporation on the nanomechanical properties of pure dipalmitoylphosphatidylcholine (DPPC) vesicles was studied using atomic force microscope (AFM) on mica surface. The vesicles were prepared by extrusion and adsorbed on the mica surface. The forces, measured between an AFM tip and the vesicle, presented that the breakthrough of the tip into the vesicles occurred two times. Each breakthrough represented each penetration of the tip into each bilayer. Force data prior to the first breakthrough were fitted well with the Hertzian model to estimate Young's modulus and bending modulus of the vesicles. It was found that the Young's modulus and bending modulus were not varied with the incorporation of AGs (streptomycins) up to the 1:1 AG/DPPC vesicle system. This result may suggest that the AGs do not lead to the disruption of DPPC packing.

Original language	English
Pages (from-to)	517-520
Number of pages	4
Journal	Colloids and Surfaces B: Biointerfaces
Volume	88
Issue number	1
DOIs	https://doi.org/10.1016/j.colsurfb.2011.07.046
State	Published - 1 Nov 2011

Keywords

Aminoglycoside
Atomic force microscope
Bending modulus
Lipid layer
Vesicle
Young modulus

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10.1016/j.colsurfb.2011.07.046

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abstract = "The effect of the aminoglycoside (streptomycin) incorporation on the nanomechanical properties of pure dipalmitoylphosphatidylcholine (DPPC) vesicles was studied using atomic force microscope (AFM) on mica surface. The vesicles were prepared by extrusion and adsorbed on the mica surface. The forces, measured between an AFM tip and the vesicle, presented that the breakthrough of the tip into the vesicles occurred two times. Each breakthrough represented each penetration of the tip into each bilayer. Force data prior to the first breakthrough were fitted well with the Hertzian model to estimate Young's modulus and bending modulus of the vesicles. It was found that the Young's modulus and bending modulus were not varied with the incorporation of AGs (streptomycins) up to the 1:1 AG/DPPC vesicle system. This result may suggest that the AGs do not lead to the disruption of DPPC packing.",

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KW - Atomic force microscope

KW - Bending modulus

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KW - Vesicle

KW - Young modulus

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Effect of aminoglycoside on mechanical properties of zwitterionic phospholipid vesicles

Abstract

Keywords

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