@inbook{8608681f49ab4b00bac3d1a4684414b3,
title = "Electrophoretic implementation of the solution-depletion method for measuring protein adsorption, adsorption kinetics, and adsorption competition among multiple proteins in solution",
abstract = "The venerable solution-depletion method is perhaps the most unambiguous method of measuring solute adsorption from solution to solid particles, requiring neither complex instrumentation nor associated interpretive theory. We describe herein an SDS-gel electrophoresis implementation of the solution-depletion method for measuring protein adsorption and protein-adsorption kinetics. Silanized-glass particles with different surface chemistry/energy and hydrophobic sepharose-based chromatographic media are used as example adsorbents. Electrophoretic separation enables quantification of adsorption competition among multiple proteins in solution for the same adsorbent surface, demonstrated herein by adsorption-competition kinetics from binary solution.",
keywords = "Adsorption competition, Adsorption kinetics, Electrophoresis, Protein adsorption, Solution depletion",
author = "Hyeran Noh and Naris Barnthip and Purnendu Parhi and Vogler, \{Erwin A.\}",
year = "2013",
doi = "10.1007/978-1-62703-462-3\_12",
language = "English",
isbn = "9781627034616",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "157--166",
booktitle = "Nanomaterial Interfaces in Biology",
}