TY - JOUR
T1 - Enhanced Osteogenic Commitment of Human Mesenchymal Stem Cells on Polyethylene Glycol-Based Cryogel with Graphene Oxide Substrate
AU - Kim, Hwan D.
AU - Kim, Jiyong
AU - Koh, Rachel H.
AU - Shim, Jimin
AU - Lee, Jong Chan
AU - Kim, Tae Il
AU - Hwang, Nathaniel S.
N1 - Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/10/9
Y1 - 2017/10/9
N2 - Graphene oxide (GO) is considered a comparatively recent biomaterial with enormous potential because of its nontoxicity, high dispersity, and enhanced interaction with biomolecules. These characteristics of GO can promote the interactions between the substrates and cell surfaces. In this study, we incorporated GO in a cryogel-based scaffold system to observe their influence on the osteogenic responses of human tonsil-derived mesenchymal stem cells (hTMSCs). Compared to polyethylene glycol (PEG)-based cryogel scaffold, GO-embedded PEG-based (PEGDA-GO) cryogels not only showed improved cell attachment and focal adhesion kinase (FAK) signaling activation but also enhanced cell viability. Taken together, we demonstrated that PEGDA-GO cryogels can stimulate osteogenic differentiation under an osteoinductive condition and enhance osteogenic phenotypes compared to the control group. In summary, we demonstrate that GO embedded in cryogels system is an effective biofunctionalizing scaffold to control osteogenic commitment of stem cells.
AB - Graphene oxide (GO) is considered a comparatively recent biomaterial with enormous potential because of its nontoxicity, high dispersity, and enhanced interaction with biomolecules. These characteristics of GO can promote the interactions between the substrates and cell surfaces. In this study, we incorporated GO in a cryogel-based scaffold system to observe their influence on the osteogenic responses of human tonsil-derived mesenchymal stem cells (hTMSCs). Compared to polyethylene glycol (PEG)-based cryogel scaffold, GO-embedded PEG-based (PEGDA-GO) cryogels not only showed improved cell attachment and focal adhesion kinase (FAK) signaling activation but also enhanced cell viability. Taken together, we demonstrated that PEGDA-GO cryogels can stimulate osteogenic differentiation under an osteoinductive condition and enhance osteogenic phenotypes compared to the control group. In summary, we demonstrate that GO embedded in cryogels system is an effective biofunctionalizing scaffold to control osteogenic commitment of stem cells.
KW - bone tissue engineering
KW - graphene oxide incorporation
KW - mesenchymal stem cells
KW - osteogenesis
KW - polyethylene glycol based cryogel
UR - https://www.scopus.com/pages/publications/85030830446
U2 - 10.1021/acsbiomaterials.7b00299
DO - 10.1021/acsbiomaterials.7b00299
M3 - Article
C2 - 33445304
AN - SCOPUS:85030830446
SN - 2373-9878
VL - 3
SP - 2470
EP - 2479
JO - ACS Biomaterials Science and Engineering
JF - ACS Biomaterials Science and Engineering
IS - 10
ER -