Gelatin-based extracellular matrix cryogels for cartilage tissue engineering

Min Eui Han; Byung Jae Kang; Su Hwan Kim; Hwan D. Kim; Nathaniel S. Hwang

doi:10.1016/j.jiec.2016.10.011

Gelatin-based extracellular matrix cryogels for cartilage tissue engineering

Min Eui Han
, Byung Jae Kang
, Su Hwan Kim
, Hwan D. Kim
, Nathaniel S. Hwang

College of Energy and Biotechnology

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

In this study, gelatin-based cryogels were fabricated by mixing methacrylated gelatin (GelMA) with methacrylated hyaluronic acid (MeHA) or methacrylated chondroitin sulfate (MeCS) for cartilage tissue engineering. In vitro revealed that MeCS incorporated gelatin-based cryogel (G-MeCS) showed significant cartilaginous tissue stimulation. Furthermore, the cell-laden gelatin-based ECM cryogels were implanted into mouse subcutaneous tissue for 6 weeks and displayed uniform distribution of cells with normal phenotype maintenance. Finally, when these cryogels were implanted into osteochondral defect of New Zealand white rabbit, full integration with host tissue and increased cellularity were observed with G-MeCS cryogel.

Original language	English
Pages (from-to)	421-429
Number of pages	9
Journal	Journal of Industrial and Engineering Chemistry
Volume	45
DOIs	https://doi.org/10.1016/j.jiec.2016.10.011
State	Published - 25 Jan 2017

Keywords

Cartilage
Chondroitin sulfate
Cryogel
Osteochondral
Tissue engineering

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10.1016/j.jiec.2016.10.011

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title = "Gelatin-based extracellular matrix cryogels for cartilage tissue engineering",

abstract = "In this study, gelatin-based cryogels were fabricated by mixing methacrylated gelatin (GelMA) with methacrylated hyaluronic acid (MeHA) or methacrylated chondroitin sulfate (MeCS) for cartilage tissue engineering. In vitro revealed that MeCS incorporated gelatin-based cryogel (G-MeCS) showed significant cartilaginous tissue stimulation. Furthermore, the cell-laden gelatin-based ECM cryogels were implanted into mouse subcutaneous tissue for 6 weeks and displayed uniform distribution of cells with normal phenotype maintenance. Finally, when these cryogels were implanted into osteochondral defect of New Zealand white rabbit, full integration with host tissue and increased cellularity were observed with G-MeCS cryogel.",

keywords = "Cartilage, Chondroitin sulfate, Cryogel, Osteochondral, Tissue engineering",

author = "Han, \{Min Eui\} and Kang, \{Byung Jae\} and Kim, \{Su Hwan\} and Kim, \{Hwan D.\} and Hwang, \{Nathaniel S.\}",

note = "Publisher Copyright: {\textcopyright} 2016 The Korean Society of Industrial and Engineering Chemistry",

year = "2017",

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day = "25",

doi = "10.1016/j.jiec.2016.10.011",

language = "English",

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journal = "Journal of Industrial and Engineering Chemistry",

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TY - JOUR

T1 - Gelatin-based extracellular matrix cryogels for cartilage tissue engineering

AU - Han, Min Eui

AU - Kang, Byung Jae

AU - Kim, Su Hwan

AU - Kim, Hwan D.

AU - Hwang, Nathaniel S.

PY - 2017/1/25

Y1 - 2017/1/25

N2 - In this study, gelatin-based cryogels were fabricated by mixing methacrylated gelatin (GelMA) with methacrylated hyaluronic acid (MeHA) or methacrylated chondroitin sulfate (MeCS) for cartilage tissue engineering. In vitro revealed that MeCS incorporated gelatin-based cryogel (G-MeCS) showed significant cartilaginous tissue stimulation. Furthermore, the cell-laden gelatin-based ECM cryogels were implanted into mouse subcutaneous tissue for 6 weeks and displayed uniform distribution of cells with normal phenotype maintenance. Finally, when these cryogels were implanted into osteochondral defect of New Zealand white rabbit, full integration with host tissue and increased cellularity were observed with G-MeCS cryogel.

AB - In this study, gelatin-based cryogels were fabricated by mixing methacrylated gelatin (GelMA) with methacrylated hyaluronic acid (MeHA) or methacrylated chondroitin sulfate (MeCS) for cartilage tissue engineering. In vitro revealed that MeCS incorporated gelatin-based cryogel (G-MeCS) showed significant cartilaginous tissue stimulation. Furthermore, the cell-laden gelatin-based ECM cryogels were implanted into mouse subcutaneous tissue for 6 weeks and displayed uniform distribution of cells with normal phenotype maintenance. Finally, when these cryogels were implanted into osteochondral defect of New Zealand white rabbit, full integration with host tissue and increased cellularity were observed with G-MeCS cryogel.

KW - Cartilage

KW - Chondroitin sulfate

KW - Cryogel

KW - Osteochondral

KW - Tissue engineering

UR - https://www.scopus.com/pages/publications/84991275514

U2 - 10.1016/j.jiec.2016.10.011

DO - 10.1016/j.jiec.2016.10.011

M3 - Article

AN - SCOPUS:84991275514

SN - 1226-086X

VL - 45

SP - 421

EP - 429

JO - Journal of Industrial and Engineering Chemistry

JF - Journal of Industrial and Engineering Chemistry

ER -

Gelatin-based extracellular matrix cryogels for cartilage tissue engineering

Abstract

Keywords

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