High-throughput single-cell electroporation microchip with three dimensional si microelectrodes for gene transfection

Y. H. Cho; S. W. Lee; B. Le Pioufle; N. Takama; S. Takeuchi; T. Fujii; B. J. Kim

doi:10.1109/SENSOR.2007.4300286

High-throughput single-cell electroporation microchip with three dimensional si microelectrodes for gene transfection

Y. H. Cho, S. W. Lee, B. Le Pioufle, N. Takama, S. Takeuchi, T. Fujii, B. J. Kim

Dept. of Mechanical System and Design Engineering

The University of Tokyo

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Scopus citations

Abstract

We present details on fabrication of electroporation microchip, practical experiments of single-cell electroporation with our fabricated microchip. Also, the continuous electroporation for the continuous flow of cells is used for high-throughput electroporation. The delivery efficiency and cell viability tests are provided and the successful GFP transfection into cells is also evaluated with a fluorescent microscope after electroporation.

Original language	English
Title of host publication	TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems
Pages	939-942
Number of pages	4
DOIs	https://doi.org/10.1109/SENSOR.2007.4300286
State	Published - 2007
Event	4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07 - Lyon, France Duration: 10 Jun 2007 → 14 Jun 2007

Publication series

Name	TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems

Conference

Conference	4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07
Country/Territory	France
City	Lyon
Period	10/06/07 → 14/06/07

Keywords

Electroporation
GFP
Twin microcantilever array

Access to Document

10.1109/SENSOR.2007.4300286

Cite this

Cho, Y. H., Lee, S. W., Le Pioufle, B., Takama, N., Takeuchi, S., Fujii, T., & Kim, B. J. (2007). High-throughput single-cell electroporation microchip with three dimensional si microelectrodes for gene transfection. In TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 939-942). Article 4300286 (TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems). https://doi.org/10.1109/SENSOR.2007.4300286

Cho, Y. H. ; Lee, S. W. ; Le Pioufle, B. et al. / High-throughput single-cell electroporation microchip with three dimensional si microelectrodes for gene transfection. TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems. 2007. pp. 939-942 (TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems).

@inproceedings{952a42af37b14eb3ad1b224547712f86,

title = "High-throughput single-cell electroporation microchip with three dimensional si microelectrodes for gene transfection",

abstract = "We present details on fabrication of electroporation microchip, practical experiments of single-cell electroporation with our fabricated microchip. Also, the continuous electroporation for the continuous flow of cells is used for high-throughput electroporation. The delivery efficiency and cell viability tests are provided and the successful GFP transfection into cells is also evaluated with a fluorescent microscope after electroporation.",

keywords = "Electroporation, GFP, Twin microcantilever array",

author = "Cho, \{Y. H.\} and Lee, \{S. W.\} and \{Le Pioufle\}, B. and N. Takama and S. Takeuchi and T. Fujii and Kim, \{B. J.\}",

year = "2007",

doi = "10.1109/SENSOR.2007.4300286",

language = "English",

isbn = "1424408423",

series = "TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems",

pages = "939--942",

booktitle = "TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems",

note = "4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07 ; Conference date: 10-06-2007 Through 14-06-2007",

}

Cho, YH, Lee, SW, Le Pioufle, B, Takama, N, Takeuchi, S, Fujii, T & Kim, BJ 2007, High-throughput single-cell electroporation microchip with three dimensional si microelectrodes for gene transfection. in TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems., 4300286, TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems, pp. 939-942, 4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07, Lyon, France, 10/06/07. https://doi.org/10.1109/SENSOR.2007.4300286

High-throughput single-cell electroporation microchip with three dimensional si microelectrodes for gene transfection. / Cho, Y. H.; Lee, S. W.; Le Pioufle, B. et al.
TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems. 2007. p. 939-942 4300286 (TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - High-throughput single-cell electroporation microchip with three dimensional si microelectrodes for gene transfection

AU - Cho, Y. H.

AU - Lee, S. W.

AU - Le Pioufle, B.

AU - Takama, N.

AU - Takeuchi, S.

AU - Fujii, T.

AU - Kim, B. J.

PY - 2007

Y1 - 2007

N2 - We present details on fabrication of electroporation microchip, practical experiments of single-cell electroporation with our fabricated microchip. Also, the continuous electroporation for the continuous flow of cells is used for high-throughput electroporation. The delivery efficiency and cell viability tests are provided and the successful GFP transfection into cells is also evaluated with a fluorescent microscope after electroporation.

AB - We present details on fabrication of electroporation microchip, practical experiments of single-cell electroporation with our fabricated microchip. Also, the continuous electroporation for the continuous flow of cells is used for high-throughput electroporation. The delivery efficiency and cell viability tests are provided and the successful GFP transfection into cells is also evaluated with a fluorescent microscope after electroporation.

KW - Electroporation

KW - GFP

KW - Twin microcantilever array

UR - http://www.scopus.com/inward/record.url?scp=50049090326&partnerID=8YFLogxK

U2 - 10.1109/SENSOR.2007.4300286

DO - 10.1109/SENSOR.2007.4300286

M3 - Conference contribution

AN - SCOPUS:50049090326

SN - 1424408423

SN - 9781424408429

T3 - TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems

SP - 939

EP - 942

BT - TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems

T2 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07

Y2 - 10 June 2007 through 14 June 2007

ER -

Cho YH, Lee SW, Le Pioufle B, Takama N, Takeuchi S, Fujii T et al. High-throughput single-cell electroporation microchip with three dimensional si microelectrodes for gene transfection. In TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems. 2007. p. 939-942. 4300286. (TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems). doi: 10.1109/SENSOR.2007.4300286

High-throughput single-cell electroporation microchip with three dimensional si microelectrodes for gene transfection

Abstract

Publication series

Conference

Keywords

Access to Document

Other files and links

Fingerprint

Cite this