High-throughput single-cell electroporation microchip with three dimensional si microelectrodes for gene transfection

Y. H. Cho; S. W. Lee; B. Le Pioufle; N. Takama; S. Takeuchi; T. Fujii; B. J. Kim

doi:10.1109/SENSOR.2007.4300286

High-throughput single-cell electroporation microchip with three dimensional si microelectrodes for gene transfection

Y. H. Cho
, S. W. Lee
, B. Le Pioufle
, N. Takama
, S. Takeuchi
, T. Fujii
, B. J. Kim

Dept. of Mechanical System and Design Engineering

The University of Tokyo

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Scopus citations

Abstract

We present details on fabrication of electroporation microchip, practical experiments of single-cell electroporation with our fabricated microchip. Also, the continuous electroporation for the continuous flow of cells is used for high-throughput electroporation. The delivery efficiency and cell viability tests are provided and the successful GFP transfection into cells is also evaluated with a fluorescent microscope after electroporation.

Original language	English
Title of host publication	TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems
Pages	939-942
Number of pages	4
DOIs	https://doi.org/10.1109/SENSOR.2007.4300286
State	Published - 2007
Event	4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07 - Lyon, France Duration: 10 Jun 2007 → 14 Jun 2007

Publication series

Name	TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems

Conference

Conference	4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07
Country/Territory	France
City	Lyon
Period	10/06/07 → 14/06/07

Keywords

Electroporation
GFP
Twin microcantilever array

Access to Document

10.1109/SENSOR.2007.4300286

Cite this

Cho, Y. H., Lee, S. W., Le Pioufle, B., Takama, N., Takeuchi, S., Fujii, T., & Kim, B. J. (2007). High-throughput single-cell electroporation microchip with three dimensional si microelectrodes for gene transfection. In TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 939-942). Article 4300286 (TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems). https://doi.org/10.1109/SENSOR.2007.4300286

Cho, Y. H. ; Lee, S. W. ; Le Pioufle, B. et al. / High-throughput single-cell electroporation microchip with three dimensional si microelectrodes for gene transfection. TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems. 2007. pp. 939-942 (TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems).

@inproceedings{952a42af37b14eb3ad1b224547712f86,

title = "High-throughput single-cell electroporation microchip with three dimensional si microelectrodes for gene transfection",

abstract = "We present details on fabrication of electroporation microchip, practical experiments of single-cell electroporation with our fabricated microchip. Also, the continuous electroporation for the continuous flow of cells is used for high-throughput electroporation. The delivery efficiency and cell viability tests are provided and the successful GFP transfection into cells is also evaluated with a fluorescent microscope after electroporation.",

keywords = "Electroporation, GFP, Twin microcantilever array",

author = "Cho, \{Y. H.\} and Lee, \{S. W.\} and \{Le Pioufle\}, B. and N. Takama and S. Takeuchi and T. Fujii and Kim, \{B. J.\}",

year = "2007",

doi = "10.1109/SENSOR.2007.4300286",

language = "English",

isbn = "1424408423",

series = "TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems",

pages = "939--942",

booktitle = "TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems",

note = "4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07 ; Conference date: 10-06-2007 Through 14-06-2007",

}

Cho, YH, Lee, SW, Le Pioufle, B, Takama, N, Takeuchi, S, Fujii, T & Kim, BJ 2007, High-throughput single-cell electroporation microchip with three dimensional si microelectrodes for gene transfection. in TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems., 4300286, TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems, pp. 939-942, 4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07, Lyon, France, 10/06/07. https://doi.org/10.1109/SENSOR.2007.4300286

High-throughput single-cell electroporation microchip with three dimensional si microelectrodes for gene transfection. / Cho, Y. H.; Lee, S. W.; Le Pioufle, B. et al.
TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems. 2007. p. 939-942 4300286 (TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - High-throughput single-cell electroporation microchip with three dimensional si microelectrodes for gene transfection

AU - Cho, Y. H.

AU - Lee, S. W.

AU - Le Pioufle, B.

AU - Takama, N.

AU - Takeuchi, S.

AU - Fujii, T.

AU - Kim, B. J.

PY - 2007

Y1 - 2007

N2 - We present details on fabrication of electroporation microchip, practical experiments of single-cell electroporation with our fabricated microchip. Also, the continuous electroporation for the continuous flow of cells is used for high-throughput electroporation. The delivery efficiency and cell viability tests are provided and the successful GFP transfection into cells is also evaluated with a fluorescent microscope after electroporation.

AB - We present details on fabrication of electroporation microchip, practical experiments of single-cell electroporation with our fabricated microchip. Also, the continuous electroporation for the continuous flow of cells is used for high-throughput electroporation. The delivery efficiency and cell viability tests are provided and the successful GFP transfection into cells is also evaluated with a fluorescent microscope after electroporation.

KW - Electroporation

KW - GFP

KW - Twin microcantilever array

UR - https://www.scopus.com/pages/publications/50049090326

U2 - 10.1109/SENSOR.2007.4300286

DO - 10.1109/SENSOR.2007.4300286

M3 - Conference contribution

AN - SCOPUS:50049090326

SN - 1424408423

SN - 9781424408429

T3 - TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems

SP - 939

EP - 942

BT - TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems

T2 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07

Y2 - 10 June 2007 through 14 June 2007

ER -

Cho YH, Lee SW, Le Pioufle B, Takama N, Takeuchi S, Fujii T et al. High-throughput single-cell electroporation microchip with three dimensional si microelectrodes for gene transfection. In TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems. 2007. p. 939-942. 4300286. (TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems). doi: 10.1109/SENSOR.2007.4300286

High-throughput single-cell electroporation microchip with three dimensional si microelectrodes for gene transfection

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Keywords

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