In Vivo Vascular Network Forming Assay

Hwan D. Kim; Ruei Zeng Lin; Juan M. Melero-Martin

doi:10.1007/978-1-0716-0916-3_14

In Vivo Vascular Network Forming Assay

Hwan D. Kim
, Ruei Zeng Lin
, Juan M. Melero-Martin

College of Energy and Biotechnology

Boston Children's Hospital
Harvard University

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

5 Scopus citations

Abstract

The capability of forming functional blood vessel networks is critical for the characterization of endothelial cells. In this chapter, we will review a modified in vivo vascular network forming assay by replacing traditional mouse tumor-derived Matrigel with a well-defined collagen–fibrin hydrogel. The assay is reliable and does not require special equipment, surgical procedure, or a skilled person to perform. Moreover, investigators can modify this method on-demand for testing different cell sources, perturbation of gene functions, growth factors, and pharmaceutical molecules, and for the development and investigation of strategies to enhance neovascularization of engineered human tissues and organs.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	193-203
Number of pages	11
DOIs	https://doi.org/10.1007/978-1-0716-0916-3_14
State	Published - 2021

Publication series

Name	Methods in Molecular Biology
Volume	2206
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Endothelial
Tissue engineering
Vascularization
Vessel formation

Access to Document

10.1007/978-1-0716-0916-3_14

Cite this

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title = "In Vivo Vascular Network Forming Assay",

abstract = "The capability of forming functional blood vessel networks is critical for the characterization of endothelial cells. In this chapter, we will review a modified in vivo vascular network forming assay by replacing traditional mouse tumor-derived Matrigel with a well-defined collagen–fibrin hydrogel. The assay is reliable and does not require special equipment, surgical procedure, or a skilled person to perform. Moreover, investigators can modify this method on-demand for testing different cell sources, perturbation of gene functions, growth factors, and pharmaceutical molecules, and for the development and investigation of strategies to enhance neovascularization of engineered human tissues and organs.",

keywords = "Endothelial, Tissue engineering, Vascularization, Vessel formation",

author = "Kim, \{Hwan D.\} and Lin, \{Ruei Zeng\} and Melero-Martin, \{Juan M.\}",

note = "Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2021",

doi = "10.1007/978-1-0716-0916-3\_14",

language = "English",

series = "Methods in Molecular Biology",

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TY - CHAP

T1 - In Vivo Vascular Network Forming Assay

AU - Kim, Hwan D.

AU - Lin, Ruei Zeng

AU - Melero-Martin, Juan M.

PY - 2021

Y1 - 2021

N2 - The capability of forming functional blood vessel networks is critical for the characterization of endothelial cells. In this chapter, we will review a modified in vivo vascular network forming assay by replacing traditional mouse tumor-derived Matrigel with a well-defined collagen–fibrin hydrogel. The assay is reliable and does not require special equipment, surgical procedure, or a skilled person to perform. Moreover, investigators can modify this method on-demand for testing different cell sources, perturbation of gene functions, growth factors, and pharmaceutical molecules, and for the development and investigation of strategies to enhance neovascularization of engineered human tissues and organs.

AB - The capability of forming functional blood vessel networks is critical for the characterization of endothelial cells. In this chapter, we will review a modified in vivo vascular network forming assay by replacing traditional mouse tumor-derived Matrigel with a well-defined collagen–fibrin hydrogel. The assay is reliable and does not require special equipment, surgical procedure, or a skilled person to perform. Moreover, investigators can modify this method on-demand for testing different cell sources, perturbation of gene functions, growth factors, and pharmaceutical molecules, and for the development and investigation of strategies to enhance neovascularization of engineered human tissues and organs.

KW - Endothelial

KW - Tissue engineering

KW - Vascularization

KW - Vessel formation

UR - https://www.scopus.com/pages/publications/85090536637

U2 - 10.1007/978-1-0716-0916-3_14

DO - 10.1007/978-1-0716-0916-3_14

M3 - Chapter

C2 - 32754819

AN - SCOPUS:85090536637

T3 - Methods in Molecular Biology

SP - 193

EP - 203

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

In Vivo Vascular Network Forming Assay

Abstract

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UN SDGs

Keywords

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