Influence of heat treatment on biological properties of Chitosan toward vascular cells in vitro

Sae Hwan Lim; Ki Sook Park; Hyun Sook Park; Yong Jae Gin; In Sup Noh; Chan Woong Park; Young Sook Son; Chun Ho Kim

doi:10.1002/masy.200550623

Influence of heat treatment on biological properties of Chitosan toward vascular cells in vitro

Sae Hwan Lim, Ki Sook Park, Hyun Sook Park, Yong Jae Gin, In Sup Noh, Chan Woong Park, Young Sook Son, Chun Ho Kim

Dept. of Chemical and Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The effects of heat treatment of chitosan film on the proliferation of vascular cells were examined in vitro. Chitosan film was heat treated at 110°C in vacuo for 1-3 days. Heat treatment slightly improved the hydrophilicity of chitosan, although the cross-linking of chitosan took place after heat treatment. Chitosan films were cytocompatible toward smooth muscle cells (SMCs) and human dermal microvascular endothelial cells (HDMECs) before and after heat treatment of chitosan. In particular, the heat treated chitosan supported the proliferation of both vascular cell types for one day. The growth of HDMECs was greater than that of SMCs on heat treated chitosan film for one day. Heat treated chitosan has intrinsic biological activity which could contribute to a retardation of SMC growth relative to HDMECs.

Original language	English
Pages (from-to)	263-274
Number of pages	12
Journal	Macromolecular Symposia
Volume	224
DOIs	https://doi.org/10.1002/masy.200550623
State	Published - Apr 2005

Keywords

Biocompatibility
Chitosan
Endothelial cells
Heat treatment
Smooth muscle cells

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10.1002/masy.200550623

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title = "Influence of heat treatment on biological properties of Chitosan toward vascular cells in vitro",

abstract = "The effects of heat treatment of chitosan film on the proliferation of vascular cells were examined in vitro. Chitosan film was heat treated at 110°C in vacuo for 1-3 days. Heat treatment slightly improved the hydrophilicity of chitosan, although the cross-linking of chitosan took place after heat treatment. Chitosan films were cytocompatible toward smooth muscle cells (SMCs) and human dermal microvascular endothelial cells (HDMECs) before and after heat treatment of chitosan. In particular, the heat treated chitosan supported the proliferation of both vascular cell types for one day. The growth of HDMECs was greater than that of SMCs on heat treated chitosan film for one day. Heat treated chitosan has intrinsic biological activity which could contribute to a retardation of SMC growth relative to HDMECs.",

keywords = "Biocompatibility, Chitosan, Endothelial cells, Heat treatment, Smooth muscle cells",

author = "Lim, \{Sae Hwan\} and Park, \{Ki Sook\} and Park, \{Hyun Sook\} and Gin, \{Yong Jae\} and Noh, \{In Sup\} and Park, \{Chan Woong\} and Son, \{Young Sook\} and Kim, \{Chun Ho\}",

year = "2005",

month = apr,

doi = "10.1002/masy.200550623",

language = "English",

volume = "224",

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journal = "Macromolecular Symposia",

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T1 - Influence of heat treatment on biological properties of Chitosan toward vascular cells in vitro

AU - Lim, Sae Hwan

AU - Park, Ki Sook

AU - Park, Hyun Sook

AU - Gin, Yong Jae

AU - Noh, In Sup

AU - Park, Chan Woong

AU - Son, Young Sook

AU - Kim, Chun Ho

PY - 2005/4

Y1 - 2005/4

N2 - The effects of heat treatment of chitosan film on the proliferation of vascular cells were examined in vitro. Chitosan film was heat treated at 110°C in vacuo for 1-3 days. Heat treatment slightly improved the hydrophilicity of chitosan, although the cross-linking of chitosan took place after heat treatment. Chitosan films were cytocompatible toward smooth muscle cells (SMCs) and human dermal microvascular endothelial cells (HDMECs) before and after heat treatment of chitosan. In particular, the heat treated chitosan supported the proliferation of both vascular cell types for one day. The growth of HDMECs was greater than that of SMCs on heat treated chitosan film for one day. Heat treated chitosan has intrinsic biological activity which could contribute to a retardation of SMC growth relative to HDMECs.

AB - The effects of heat treatment of chitosan film on the proliferation of vascular cells were examined in vitro. Chitosan film was heat treated at 110°C in vacuo for 1-3 days. Heat treatment slightly improved the hydrophilicity of chitosan, although the cross-linking of chitosan took place after heat treatment. Chitosan films were cytocompatible toward smooth muscle cells (SMCs) and human dermal microvascular endothelial cells (HDMECs) before and after heat treatment of chitosan. In particular, the heat treated chitosan supported the proliferation of both vascular cell types for one day. The growth of HDMECs was greater than that of SMCs on heat treated chitosan film for one day. Heat treated chitosan has intrinsic biological activity which could contribute to a retardation of SMC growth relative to HDMECs.

KW - Biocompatibility

KW - Chitosan

KW - Endothelial cells

KW - Heat treatment

KW - Smooth muscle cells

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U2 - 10.1002/masy.200550623

DO - 10.1002/masy.200550623

M3 - Article

AN - SCOPUS:20944446432

SN - 1022-1360

VL - 224

SP - 263

EP - 274

JO - Macromolecular Symposia

JF - Macromolecular Symposia

ER -

Influence of heat treatment on biological properties of Chitosan toward vascular cells in vitro

Abstract

Keywords

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