Inhibitory effects of transglycoslyation products of soy isoflavones on cholesterol biosynthesis

Lang Kuk Yoo; Seung Jun Choi; Tae Wha Moon; Jae Hoon Shim

doi:10.3746/jkfn.2016.45.2.293

Inhibitory effects of transglycoslyation products of soy isoflavones on cholesterol biosynthesis

Lang Kuk Yoo, Seung Jun Choi, Tae Wha Moon, Jae Hoon Shim

Dept. of Food Science and Technology

Research output: Contribution to journal › Article › peer-review

Abstract

Hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reductase) is the rate-limiting enzyme in biosynthesis of cholesterol in animals. In this study, inhibitory effects of isoflavone glycosides on HMG-CoA reductase were investigated. At sample concentration of 100 μM, genistein-7-O-triglucoside (G2-genistin) inhibited HMG-CoA reductase activity by approximately 18%, whereas daidzein-7-O-triglucoside had no inhibitory effect. In the kinetic experiments with Syrian hamster HMG-CoA reductase, G2-genistin showed inhibitory efficacy with an invariable V_max value, suggesting that G2-genistin works as a competitive inhibitor of HMG-CoA reductase and has potential for hypocholesterolemic action through direct regulation of HMG-CoA reductase.

Original language	English
Pages (from-to)	293-297
Number of pages	5
Journal	Journal of the Korean Society of Food Science and Nutrition
Volume	45
Issue number	2
DOIs	https://doi.org/10.3746/jkfn.2016.45.2.293
State	Published - Feb 2016

Keywords

Cholesterol biosynthesis
HMG-CoA reductase
Isoflavone
Isoflavone glycoside
Soybean

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10.3746/jkfn.2016.45.2.293

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abstract = "Hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reductase) is the rate-limiting enzyme in biosynthesis of cholesterol in animals. In this study, inhibitory effects of isoflavone glycosides on HMG-CoA reductase were investigated. At sample concentration of 100 μM, genistein-7-O-triglucoside (G2-genistin) inhibited HMG-CoA reductase activity by approximately 18\%, whereas daidzein-7-O-triglucoside had no inhibitory effect. In the kinetic experiments with Syrian hamster HMG-CoA reductase, G2-genistin showed inhibitory efficacy with an invariable Vmax value, suggesting that G2-genistin works as a competitive inhibitor of HMG-CoA reductase and has potential for hypocholesterolemic action through direct regulation of HMG-CoA reductase.",

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AU - Yoo, Lang Kuk

AU - Choi, Seung Jun

AU - Moon, Tae Wha

AU - Shim, Jae Hoon

PY - 2016/2

Y1 - 2016/2

N2 - Hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reductase) is the rate-limiting enzyme in biosynthesis of cholesterol in animals. In this study, inhibitory effects of isoflavone glycosides on HMG-CoA reductase were investigated. At sample concentration of 100 μM, genistein-7-O-triglucoside (G2-genistin) inhibited HMG-CoA reductase activity by approximately 18%, whereas daidzein-7-O-triglucoside had no inhibitory effect. In the kinetic experiments with Syrian hamster HMG-CoA reductase, G2-genistin showed inhibitory efficacy with an invariable Vmax value, suggesting that G2-genistin works as a competitive inhibitor of HMG-CoA reductase and has potential for hypocholesterolemic action through direct regulation of HMG-CoA reductase.

AB - Hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reductase) is the rate-limiting enzyme in biosynthesis of cholesterol in animals. In this study, inhibitory effects of isoflavone glycosides on HMG-CoA reductase were investigated. At sample concentration of 100 μM, genistein-7-O-triglucoside (G2-genistin) inhibited HMG-CoA reductase activity by approximately 18%, whereas daidzein-7-O-triglucoside had no inhibitory effect. In the kinetic experiments with Syrian hamster HMG-CoA reductase, G2-genistin showed inhibitory efficacy with an invariable Vmax value, suggesting that G2-genistin works as a competitive inhibitor of HMG-CoA reductase and has potential for hypocholesterolemic action through direct regulation of HMG-CoA reductase.

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KW - HMG-CoA reductase

KW - Isoflavone

KW - Isoflavone glycoside

KW - Soybean

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Inhibitory effects of transglycoslyation products of soy isoflavones on cholesterol biosynthesis

Abstract

Keywords

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