TY - JOUR
T1 - Intestinal anti-inflammatory effects of cinnamon extracts in a co-culture model of intestinal epithelial Caco-2 cells and RAW264.7 macrophages
AU - Kim, Min Seo
AU - Kim, Ji Yeon
N1 - Publisher Copyright:
© 2017, The Korean Society for Applied Biological Chemistry.
PY - 2017/10/1
Y1 - 2017/10/1
N2 - Cinnamon is the bark of Cinnamomum japonicum SIEB, which possesses anti-microbial, anti-tyrosinase, and antioxidant activities. However, studies on the intestinal health benefits of cinnamon are scarce. Therefore, we were aimed at assessing the potential anti-inflammatory action of cinnamon extracts in a cellular model of intestinal inflammation. Cinnamon extracts were prepared using hot water extract and 70% ethanol extract. Prior to inducing inflammation, co-cultured Caco-2 cells with RAW264.7 cells were treated with the cinnamon extracts. Lipopolysaccharide (LPS) was treated to induce inflammation in RAW264.7 cells. Compared to LPS-treated groups, the cells treated with cinnamon extracts maintained high transepithelial electrical resistance and decreased tight junction permeability, similar to that shown by control Caco-2 cells. In addition, cinnamon extract-treated cells showed decreased levels of inflammatory cytokines, namely nitrite (NO), PGE2, interleukin (IL)-6, IL-8, and TNF-α. These results show that cinnamon may serve as a probable agent for the development of functional supplements for maintenance of gastrointestinal health.
AB - Cinnamon is the bark of Cinnamomum japonicum SIEB, which possesses anti-microbial, anti-tyrosinase, and antioxidant activities. However, studies on the intestinal health benefits of cinnamon are scarce. Therefore, we were aimed at assessing the potential anti-inflammatory action of cinnamon extracts in a cellular model of intestinal inflammation. Cinnamon extracts were prepared using hot water extract and 70% ethanol extract. Prior to inducing inflammation, co-cultured Caco-2 cells with RAW264.7 cells were treated with the cinnamon extracts. Lipopolysaccharide (LPS) was treated to induce inflammation in RAW264.7 cells. Compared to LPS-treated groups, the cells treated with cinnamon extracts maintained high transepithelial electrical resistance and decreased tight junction permeability, similar to that shown by control Caco-2 cells. In addition, cinnamon extract-treated cells showed decreased levels of inflammatory cytokines, namely nitrite (NO), PGE2, interleukin (IL)-6, IL-8, and TNF-α. These results show that cinnamon may serve as a probable agent for the development of functional supplements for maintenance of gastrointestinal health.
KW - Caco-2
KW - Cinnamon
KW - Co-culture
KW - Gut barrier integrity
KW - Gut inflammation
UR - https://www.scopus.com/pages/publications/85027719499
U2 - 10.1007/s13765-017-0311-y
DO - 10.1007/s13765-017-0311-y
M3 - Article
AN - SCOPUS:85027719499
SN - 2468-0834
VL - 60
SP - 553
EP - 561
JO - Applied Biological Chemistry
JF - Applied Biological Chemistry
IS - 5
ER -