Long-term and stable antimicrobial properties of immobilized Ni/TiO₂ nanocomposites against Escherichia coli, Legionella thermalis, and MS2 bacteriophage

Nickel has been extensively used as a high work function metal because of its abundance, low cost, relatively non-toxic nature, and environmentally benign characteristics. However, it has rarely been extended in a form of immobilized composite, which is a practical strategy applicable for photocatalytic antimicrobial activities. In this study, a composite of nickel and TiO₂ (Ni/TiO₂) was prepared using a photodeposition method, and its antibacterial properties were investigated using Escherichia coli (E. coli). To optimize Ni/TiO₂ synthesis, the effect of various photodeposition conditions on antibacterial performance were investigated, such as the light irradiation time, metal content, TiO₂ crystalline structure, and presence or absence of electron donors (i.e., methanol). The optimized 2 wt% Ni/TiO₂ exhibited an antibacterial efficiency of 3.74 log within 7 min, which is more than 10-fold higher than that of pristine TiO₂ (2.54 log). Based on this optimized weight ratio, Ni/TiO₂ was immobilized on a steel mesh using an electrospray/thermal compression method, and its antibacterial performance was further assessed against E. coli, MS2 bacteriophage virus (MS2 phage), and a common pulmonary pathogen (Legionella thermalis, L. thermalis). Within 70 min, all target microorganisms achieved an inactivation that exceeded 4 log. Furthermore, the long-term stability and sustainable usability of the Ni/TiO₂ mesh were confirmed by performing more than 50 antibacterial evaluation cycles using E. coli. The results of this study facilitate the successful utilization of immobilized Ni/TiO₂ mesh in water disinfection applications.