TY - JOUR
T1 - Microfluidic assembly of mono-dispersed liposome and its surface modification for enhancing the colloidal stability
AU - Jo, Minje
AU - Park, Kyung Min
AU - Park, Jun Young
AU - Yu, Hyunjong
AU - Choi, Seung Jun
AU - Chang, Pahn Shick
N1 - Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2020/2/5
Y1 - 2020/2/5
N2 - In the present study, mono-dispersed 100 nm-sized liposomes (DPPC:cholesterol = 8:2 (mol)) were produced by a novel microfluidic assembly method with optimized flow rate ratio (non-aqueous phase:aqueous phase = 60.0:6.0 (mL/h)). These liposomes were incorporated with ionic surfactants, such as anionic palmitic acid (PAL) or cationic hexadecylamine (HDA), to enhance their colloidal stability, which was confirmed through TEM and ζ-potential analysis. Moreover, to enhance their intestinal stability, the surfaces of liposomes were modified with biodegradable polymers by electrostatic attraction. Specifically, cationic chitosan was coated onto anionic PAL-incorporated liposomes, whereas anionic pectin was used for cationic HDA-incorporated liposomes. Finally, branched-chain amino acids (BCAAs) were encapsulated into the PAL-incorporated liposomes with surface modifications, showing 31.2 % of encapsulation efficiency. This study could contribute a novel approach for microfluidic assembly of the liposomes, providing deeper insight into surface modifications and enhancement of the colloidal stability.
AB - In the present study, mono-dispersed 100 nm-sized liposomes (DPPC:cholesterol = 8:2 (mol)) were produced by a novel microfluidic assembly method with optimized flow rate ratio (non-aqueous phase:aqueous phase = 60.0:6.0 (mL/h)). These liposomes were incorporated with ionic surfactants, such as anionic palmitic acid (PAL) or cationic hexadecylamine (HDA), to enhance their colloidal stability, which was confirmed through TEM and ζ-potential analysis. Moreover, to enhance their intestinal stability, the surfaces of liposomes were modified with biodegradable polymers by electrostatic attraction. Specifically, cationic chitosan was coated onto anionic PAL-incorporated liposomes, whereas anionic pectin was used for cationic HDA-incorporated liposomes. Finally, branched-chain amino acids (BCAAs) were encapsulated into the PAL-incorporated liposomes with surface modifications, showing 31.2 % of encapsulation efficiency. This study could contribute a novel approach for microfluidic assembly of the liposomes, providing deeper insight into surface modifications and enhancement of the colloidal stability.
KW - Biodegradable polymer
KW - Colloidal stability
KW - Ionic surfactant
KW - Liposome
KW - Microfluidic assembly
KW - Surface modification
UR - http://www.scopus.com/inward/record.url?scp=85075859894&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfa.2019.124202
DO - 10.1016/j.colsurfa.2019.124202
M3 - Article
AN - SCOPUS:85075859894
SN - 0927-7757
VL - 586
JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects
JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects
M1 - 124202
ER -