Multiplexed Detection of Foodborne Pathogens from Contaminated Lettuces Using a Handheld Multistep Lateral Flow Assay Device

Joong Ho Shin; Jisoo Hong; Hyeyun Go; Juhwan Park; Minsuk Kong; Sangryeol Ryu; Kwang Pyo Kim; Eunjung Roh; Je Kyun Park

doi:10.1021/acs.jafc.7b03582

Multiplexed Detection of Foodborne Pathogens from Contaminated Lettuces Using a Handheld Multistep Lateral Flow Assay Device

Joong Ho Shin, Jisoo Hong, Hyeyun Go, Juhwan Park, Minsuk Kong, Sangryeol Ryu, Kwang Pyo Kim, Eunjung Roh, Je Kyun Park

Dept. of Food Science and Technology

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

This paper presents a handheld device that is capable of simplifying multistep assays to perform sensitive detection of foodborne pathogens. The device is capable of multiplexed detection of Escherichia coli (E. coli) O157:H7, Salmonella Typhimurium (S. Typhimurium), Staphylococcus aureus, and Bacillus cereus. The limit of detection for each bacterium was characterized, and then, the detection of bacteria from contaminated fresh lettuces was demonstrated for two representative foodborne pathogens. We employed a sample pretreatment protocol to recover and concentrate target bacteria from contaminated lettuces, which can detect 1.87 × 10⁴ CFU of E. coli O157:H7 and 1.47 × 10⁴ CFU of S. Typhimurium/1 g of lettuce without an enrichment process. Lastly, we demonstrated that the limit of detection can be reduced to 1 CFU of E. coli O157:H7 and 1 CFU of S. Typhimurium/1 g of lettuce by including a 6 h enrichment of contaminated lettuces in growth media before pretreatment.

Original language	English
Pages (from-to)	290-297
Number of pages	8
Journal	Journal of Agricultural and Food Chemistry
Volume	66
Issue number	1
DOIs	https://doi.org/10.1021/acs.jafc.7b03582
State	Published - 10 Jan 2018

Keywords

contaminated lettuces
foodborne pathogens
handheld device
lateral flow assay
multiplexed detection

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10.1021/acs.jafc.7b03582

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title = "Multiplexed Detection of Foodborne Pathogens from Contaminated Lettuces Using a Handheld Multistep Lateral Flow Assay Device",

abstract = "This paper presents a handheld device that is capable of simplifying multistep assays to perform sensitive detection of foodborne pathogens. The device is capable of multiplexed detection of Escherichia coli (E. coli) O157:H7, Salmonella Typhimurium (S. Typhimurium), Staphylococcus aureus, and Bacillus cereus. The limit of detection for each bacterium was characterized, and then, the detection of bacteria from contaminated fresh lettuces was demonstrated for two representative foodborne pathogens. We employed a sample pretreatment protocol to recover and concentrate target bacteria from contaminated lettuces, which can detect 1.87 × 104 CFU of E. coli O157:H7 and 1.47 × 104 CFU of S. Typhimurium/1 g of lettuce without an enrichment process. Lastly, we demonstrated that the limit of detection can be reduced to 1 CFU of E. coli O157:H7 and 1 CFU of S. Typhimurium/1 g of lettuce by including a 6 h enrichment of contaminated lettuces in growth media before pretreatment.",

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author = "Shin, \{Joong Ho\} and Jisoo Hong and Hyeyun Go and Juhwan Park and Minsuk Kong and Sangryeol Ryu and Kim, \{Kwang Pyo\} and Eunjung Roh and Park, \{Je Kyun\}",

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doi = "10.1021/acs.jafc.7b03582",

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AU - Shin, Joong Ho

AU - Hong, Jisoo

AU - Go, Hyeyun

AU - Park, Juhwan

AU - Kong, Minsuk

AU - Ryu, Sangryeol

AU - Kim, Kwang Pyo

AU - Roh, Eunjung

AU - Park, Je Kyun

PY - 2018/1/10

Y1 - 2018/1/10

N2 - This paper presents a handheld device that is capable of simplifying multistep assays to perform sensitive detection of foodborne pathogens. The device is capable of multiplexed detection of Escherichia coli (E. coli) O157:H7, Salmonella Typhimurium (S. Typhimurium), Staphylococcus aureus, and Bacillus cereus. The limit of detection for each bacterium was characterized, and then, the detection of bacteria from contaminated fresh lettuces was demonstrated for two representative foodborne pathogens. We employed a sample pretreatment protocol to recover and concentrate target bacteria from contaminated lettuces, which can detect 1.87 × 104 CFU of E. coli O157:H7 and 1.47 × 104 CFU of S. Typhimurium/1 g of lettuce without an enrichment process. Lastly, we demonstrated that the limit of detection can be reduced to 1 CFU of E. coli O157:H7 and 1 CFU of S. Typhimurium/1 g of lettuce by including a 6 h enrichment of contaminated lettuces in growth media before pretreatment.

AB - This paper presents a handheld device that is capable of simplifying multistep assays to perform sensitive detection of foodborne pathogens. The device is capable of multiplexed detection of Escherichia coli (E. coli) O157:H7, Salmonella Typhimurium (S. Typhimurium), Staphylococcus aureus, and Bacillus cereus. The limit of detection for each bacterium was characterized, and then, the detection of bacteria from contaminated fresh lettuces was demonstrated for two representative foodborne pathogens. We employed a sample pretreatment protocol to recover and concentrate target bacteria from contaminated lettuces, which can detect 1.87 × 104 CFU of E. coli O157:H7 and 1.47 × 104 CFU of S. Typhimurium/1 g of lettuce without an enrichment process. Lastly, we demonstrated that the limit of detection can be reduced to 1 CFU of E. coli O157:H7 and 1 CFU of S. Typhimurium/1 g of lettuce by including a 6 h enrichment of contaminated lettuces in growth media before pretreatment.

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Multiplexed Detection of Foodborne Pathogens from Contaminated Lettuces Using a Handheld Multistep Lateral Flow Assay Device

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