Non-invasive monitoring of hepatocellular carcinoma in transgenic mouse with bioluminescent imaging

Ju Hui Park; Kwang Il Kim; Yong Jin Lee; Tae Sup Lee; Kyeong Min Kim; Sang Soep Nahm; Young Seo Park; Gi Jeong Cheon; Sang Moo Lim; Joo Hyun Kang

doi:10.1016/j.canlet.2011.06.013

Non-invasive monitoring of hepatocellular carcinoma in transgenic mouse with bioluminescent imaging

Ju Hui Park
, Kwang Il Kim
, Yong Jin Lee
, Tae Sup Lee
, Kyeong Min Kim
, Sang Soep Nahm
, Young Seo Park
, Gi Jeong Cheon
, Sang Moo Lim
, Joo Hyun Kang

Dept. of Biomedical Sciences

Korea Institute of Radiological and Medical Sciences
Gachon University
Konkuk University

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

A small animal imaging system for hepatocellular carcinoma (HCC)-specific reporter gene expression will enable monitoring of carcinogenesis or therapeutic intervention in vivo. Transgenic mouse was developed in which firefly luciferase (fLuc) expression was controlled by the AFP enhancer/promoter. The bioluminescent signals of the transgenic neonates were strong at their liver region and decreased after birth. Bioluminescent imaging (BLI) of a transgenic mouse treated with N-nitrosodiethylamine revealed distinct fLuc activity in the liver and an increased pattern with time. The transgenic mouse model can be used to monitor AFP producing HCC by a chemical carcinogen in a live animal by BLI.

Original language	English
Pages (from-to)	53-60
Number of pages	8
Journal	Cancer Letters
Volume	310
Issue number	1
DOIs	https://doi.org/10.1016/j.canlet.2011.06.013
State	Published - 1 Nov 2011

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

α-Fetoprotein
Hepatocellular carcinoma
Luciferase
Molecular imaging
Transgenic mouse

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10.1016/j.canlet.2011.06.013

Cite this

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title = "Non-invasive monitoring of hepatocellular carcinoma in transgenic mouse with bioluminescent imaging",

abstract = "A small animal imaging system for hepatocellular carcinoma (HCC)-specific reporter gene expression will enable monitoring of carcinogenesis or therapeutic intervention in vivo. Transgenic mouse was developed in which firefly luciferase (fLuc) expression was controlled by the AFP enhancer/promoter. The bioluminescent signals of the transgenic neonates were strong at their liver region and decreased after birth. Bioluminescent imaging (BLI) of a transgenic mouse treated with N-nitrosodiethylamine revealed distinct fLuc activity in the liver and an increased pattern with time. The transgenic mouse model can be used to monitor AFP producing HCC by a chemical carcinogen in a live animal by BLI.",

keywords = "α-Fetoprotein, Hepatocellular carcinoma, Luciferase, Molecular imaging, Transgenic mouse",

author = "Park, \{Ju Hui\} and Kim, \{Kwang Il\} and Lee, \{Yong Jin\} and Lee, \{Tae Sup\} and Kim, \{Kyeong Min\} and Nahm, \{Sang Soep\} and Park, \{Young Seo\} and Cheon, \{Gi Jeong\} and Lim, \{Sang Moo\} and Kang, \{Joo Hyun\}",

year = "2011",

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doi = "10.1016/j.canlet.2011.06.013",

language = "English",

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journal = "Cancer Letters",

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T1 - Non-invasive monitoring of hepatocellular carcinoma in transgenic mouse with bioluminescent imaging

AU - Park, Ju Hui

AU - Kim, Kwang Il

AU - Lee, Yong Jin

AU - Lee, Tae Sup

AU - Kim, Kyeong Min

AU - Nahm, Sang Soep

AU - Park, Young Seo

AU - Cheon, Gi Jeong

AU - Lim, Sang Moo

AU - Kang, Joo Hyun

PY - 2011/11/1

Y1 - 2011/11/1

N2 - A small animal imaging system for hepatocellular carcinoma (HCC)-specific reporter gene expression will enable monitoring of carcinogenesis or therapeutic intervention in vivo. Transgenic mouse was developed in which firefly luciferase (fLuc) expression was controlled by the AFP enhancer/promoter. The bioluminescent signals of the transgenic neonates were strong at their liver region and decreased after birth. Bioluminescent imaging (BLI) of a transgenic mouse treated with N-nitrosodiethylamine revealed distinct fLuc activity in the liver and an increased pattern with time. The transgenic mouse model can be used to monitor AFP producing HCC by a chemical carcinogen in a live animal by BLI.

AB - A small animal imaging system for hepatocellular carcinoma (HCC)-specific reporter gene expression will enable monitoring of carcinogenesis or therapeutic intervention in vivo. Transgenic mouse was developed in which firefly luciferase (fLuc) expression was controlled by the AFP enhancer/promoter. The bioluminescent signals of the transgenic neonates were strong at their liver region and decreased after birth. Bioluminescent imaging (BLI) of a transgenic mouse treated with N-nitrosodiethylamine revealed distinct fLuc activity in the liver and an increased pattern with time. The transgenic mouse model can be used to monitor AFP producing HCC by a chemical carcinogen in a live animal by BLI.

KW - α-Fetoprotein

KW - Hepatocellular carcinoma

KW - Luciferase

KW - Molecular imaging

KW - Transgenic mouse

UR - https://www.scopus.com/pages/publications/79961126545

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DO - 10.1016/j.canlet.2011.06.013

M3 - Article

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AN - SCOPUS:79961126545

SN - 0304-3835

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SP - 53

EP - 60

JO - Cancer Letters

JF - Cancer Letters

IS - 1

ER -

Non-invasive monitoring of hepatocellular carcinoma in transgenic mouse with bioluminescent imaging

Abstract

UN SDGs

Keywords

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