One-dimensional dipolar-shift spectroscopy under magic angle spinning to determine the chemical-shift anisotropy tensors

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Abstract

An efficient one-dimensional dipolar-shift solid-state NMR method is reported that allows the characterization of chemical-shift anisotropy (CSA) and heteronuclear dipolar coupling tensors from powder samples. A combination of a multiple pulse sequence and magic angle spinning (MAS) is used to completely suppress the homonuclear 1H-1H dipolar couplings and to recover most of the 1H-X dipolar coupling along with the CSA interaction of the X nuclei (where X is 15N or 13C or any other less-sensitive nucleus directly bonded to 1H). This method is quick, simple to implement, and the results are accurate and easy to interpret. The efficacy of this method is demonstrated on a powder sample of a model peptide, n-acetyl-15N-D,L-valine, under various spinning speeds.

Original languageEnglish
Pages (from-to)20-24
Number of pages5
JournalChemical Physics Letters
Volume324
Issue number1-3
DOIs
StatePublished - 30 Jun 2000

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