TY - JOUR
T1 - Osteoblast differentiation stimulating activity of biflavonoids from Cephalotaxus koreana
AU - Lee, Mi Kyeong
AU - Lim, Song Won
AU - Yang, Hyekyung
AU - Sung, Sang Hyun
AU - Lee, Heum Sook
AU - Park, Mi Jung
AU - Kim, Young Choong
PY - 2006/6/1
Y1 - 2006/6/1
N2 - Six amentoflavone-type biflavonoids, bilobetin (1), ginkgetin (2), 4′,7″-di-O-methyl-amentoflavone (3), 7-O-methyl-isoginkgetin (4), sciadopitysin (5), and 7,4′,7″,4‴-O-methyl-amentoflavone (6), were isolated from the EtOAc fraction of Cephalotaxus koreana Nakai (Cephalotaxaceae) by bioactivity-guided fractionation technique using primary cultures of mouse osteoblasts as an in vitro assay system. Among the six biflavonoids isolated, bilobetin (1), sciadopitysin (5), and 7,4′,7″,4‴-O-methyl-amentoflavone (6) significantly increased osteoblast differentiation as assessed by alkaline phosphatase activity, collagen synthesis, and mineralization. Considering structure-activity relationship, methoxyl groups at 4′ and 4‴ in the B rings in amentoflavone-type biflavonoid might be important in osteoblast differentiation. Taken together, our present study suggests therapeutic potential of biflavonoids against bone diseases such as osteoporosis.
AB - Six amentoflavone-type biflavonoids, bilobetin (1), ginkgetin (2), 4′,7″-di-O-methyl-amentoflavone (3), 7-O-methyl-isoginkgetin (4), sciadopitysin (5), and 7,4′,7″,4‴-O-methyl-amentoflavone (6), were isolated from the EtOAc fraction of Cephalotaxus koreana Nakai (Cephalotaxaceae) by bioactivity-guided fractionation technique using primary cultures of mouse osteoblasts as an in vitro assay system. Among the six biflavonoids isolated, bilobetin (1), sciadopitysin (5), and 7,4′,7″,4‴-O-methyl-amentoflavone (6) significantly increased osteoblast differentiation as assessed by alkaline phosphatase activity, collagen synthesis, and mineralization. Considering structure-activity relationship, methoxyl groups at 4′ and 4‴ in the B rings in amentoflavone-type biflavonoid might be important in osteoblast differentiation. Taken together, our present study suggests therapeutic potential of biflavonoids against bone diseases such as osteoporosis.
KW - Alkaline phosphatase
KW - Amentoflavone-type biflavonoid
KW - Cephalotaxus koreana
KW - Collagen
KW - Mineralization
KW - Osteoblast differentiation
KW - Osteoporosis
UR - https://www.scopus.com/pages/publications/33646027558
U2 - 10.1016/j.bmcl.2006.03.018
DO - 10.1016/j.bmcl.2006.03.018
M3 - Article
C2 - 16574412
AN - SCOPUS:33646027558
SN - 0960-894X
VL - 16
SP - 2850
EP - 2854
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
IS - 11
ER -