Osteoblast differentiation stimulating activity of biflavonoids from Cephalotaxus koreana

Mi Kyeong Lee; Song Won Lim; Hyekyung Yang; Sang Hyun Sung; Heum Sook Lee; Mi Jung Park; Young Choong Kim

doi:10.1016/j.bmcl.2006.03.018

Osteoblast differentiation stimulating activity of biflavonoids from Cephalotaxus koreana

Mi Kyeong Lee
, Song Won Lim
, Hyekyung Yang
, Sang Hyun Sung
, Heum Sook Lee
, Mi Jung Park
, Young Choong Kim

Dept. of Optometry

Seoul National University
Seoul National University of Science and Technology (SNUST)

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

Six amentoflavone-type biflavonoids, bilobetin (1), ginkgetin (2), 4′,7″-di-O-methyl-amentoflavone (3), 7-O-methyl-isoginkgetin (4), sciadopitysin (5), and 7,4′,7″,4‴-O-methyl-amentoflavone (6), were isolated from the EtOAc fraction of Cephalotaxus koreana Nakai (Cephalotaxaceae) by bioactivity-guided fractionation technique using primary cultures of mouse osteoblasts as an in vitro assay system. Among the six biflavonoids isolated, bilobetin (1), sciadopitysin (5), and 7,4′,7″,4‴-O-methyl-amentoflavone (6) significantly increased osteoblast differentiation as assessed by alkaline phosphatase activity, collagen synthesis, and mineralization. Considering structure-activity relationship, methoxyl groups at 4′ and 4‴ in the B rings in amentoflavone-type biflavonoid might be important in osteoblast differentiation. Taken together, our present study suggests therapeutic potential of biflavonoids against bone diseases such as osteoporosis.

Original language	English
Pages (from-to)	2850-2854
Number of pages	5
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	16
Issue number	11
DOIs	https://doi.org/10.1016/j.bmcl.2006.03.018
State	Published - 1 Jun 2006

Keywords

Alkaline phosphatase
Amentoflavone-type biflavonoid
Cephalotaxus koreana
Collagen
Mineralization
Osteoblast differentiation
Osteoporosis

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10.1016/j.bmcl.2006.03.018

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title = "Osteoblast differentiation stimulating activity of biflavonoids from Cephalotaxus koreana",

abstract = "Six amentoflavone-type biflavonoids, bilobetin (1), ginkgetin (2), 4′,7″-di-O-methyl-amentoflavone (3), 7-O-methyl-isoginkgetin (4), sciadopitysin (5), and 7,4′,7″,4‴-O-methyl-amentoflavone (6), were isolated from the EtOAc fraction of Cephalotaxus koreana Nakai (Cephalotaxaceae) by bioactivity-guided fractionation technique using primary cultures of mouse osteoblasts as an in vitro assay system. Among the six biflavonoids isolated, bilobetin (1), sciadopitysin (5), and 7,4′,7″,4‴-O-methyl-amentoflavone (6) significantly increased osteoblast differentiation as assessed by alkaline phosphatase activity, collagen synthesis, and mineralization. Considering structure-activity relationship, methoxyl groups at 4′ and 4‴ in the B rings in amentoflavone-type biflavonoid might be important in osteoblast differentiation. Taken together, our present study suggests therapeutic potential of biflavonoids against bone diseases such as osteoporosis.",

keywords = "Alkaline phosphatase, Amentoflavone-type biflavonoid, Cephalotaxus koreana, Collagen, Mineralization, Osteoblast differentiation, Osteoporosis",

author = "Lee, \{Mi Kyeong\} and Lim, \{Song Won\} and Hyekyung Yang and Sung, \{Sang Hyun\} and Lee, \{Heum Sook\} and Park, \{Mi Jung\} and Kim, \{Young Choong\}",

year = "2006",

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doi = "10.1016/j.bmcl.2006.03.018",

language = "English",

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pages = "2850--2854",

journal = "Bioorganic and Medicinal Chemistry Letters",

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TY - JOUR

T1 - Osteoblast differentiation stimulating activity of biflavonoids from Cephalotaxus koreana

AU - Lee, Mi Kyeong

AU - Lim, Song Won

AU - Yang, Hyekyung

AU - Sung, Sang Hyun

AU - Lee, Heum Sook

AU - Park, Mi Jung

AU - Kim, Young Choong

PY - 2006/6/1

Y1 - 2006/6/1

N2 - Six amentoflavone-type biflavonoids, bilobetin (1), ginkgetin (2), 4′,7″-di-O-methyl-amentoflavone (3), 7-O-methyl-isoginkgetin (4), sciadopitysin (5), and 7,4′,7″,4‴-O-methyl-amentoflavone (6), were isolated from the EtOAc fraction of Cephalotaxus koreana Nakai (Cephalotaxaceae) by bioactivity-guided fractionation technique using primary cultures of mouse osteoblasts as an in vitro assay system. Among the six biflavonoids isolated, bilobetin (1), sciadopitysin (5), and 7,4′,7″,4‴-O-methyl-amentoflavone (6) significantly increased osteoblast differentiation as assessed by alkaline phosphatase activity, collagen synthesis, and mineralization. Considering structure-activity relationship, methoxyl groups at 4′ and 4‴ in the B rings in amentoflavone-type biflavonoid might be important in osteoblast differentiation. Taken together, our present study suggests therapeutic potential of biflavonoids against bone diseases such as osteoporosis.

AB - Six amentoflavone-type biflavonoids, bilobetin (1), ginkgetin (2), 4′,7″-di-O-methyl-amentoflavone (3), 7-O-methyl-isoginkgetin (4), sciadopitysin (5), and 7,4′,7″,4‴-O-methyl-amentoflavone (6), were isolated from the EtOAc fraction of Cephalotaxus koreana Nakai (Cephalotaxaceae) by bioactivity-guided fractionation technique using primary cultures of mouse osteoblasts as an in vitro assay system. Among the six biflavonoids isolated, bilobetin (1), sciadopitysin (5), and 7,4′,7″,4‴-O-methyl-amentoflavone (6) significantly increased osteoblast differentiation as assessed by alkaline phosphatase activity, collagen synthesis, and mineralization. Considering structure-activity relationship, methoxyl groups at 4′ and 4‴ in the B rings in amentoflavone-type biflavonoid might be important in osteoblast differentiation. Taken together, our present study suggests therapeutic potential of biflavonoids against bone diseases such as osteoporosis.

KW - Alkaline phosphatase

KW - Amentoflavone-type biflavonoid

KW - Cephalotaxus koreana

KW - Collagen

KW - Mineralization

KW - Osteoblast differentiation

KW - Osteoporosis

UR - https://www.scopus.com/pages/publications/33646027558

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DO - 10.1016/j.bmcl.2006.03.018

M3 - Article

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AN - SCOPUS:33646027558

SN - 0960-894X

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EP - 2854

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 11

ER -

Osteoblast differentiation stimulating activity of biflavonoids from Cephalotaxus koreana

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