Precision genome engineering through adenine base editing in plants

Beum Chang Kang; Jae Young Yun; Sang Tae Kim; You Jin Shin; Jahee Ryu; Minkyung Choi; Je Wook Woo; Jin Soo Kim

doi:10.1038/s41477-018-0178-x

Precision genome engineering through adenine base editing in plants

Beum Chang Kang
, Jae Young Yun
, Sang Tae Kim
, You Jin Shin
, Jahee Ryu
, Minkyung Choi
, Je Wook Woo
, Jin Soo Kim

School of Convergence

Research output: Contribution to journal › Article › peer-review

270 Scopus citations

Abstract

The recent development of adenine base editors (ABEs) has enabled efficient and precise A-to-G base conversions in higher eukaryotic cells. Here, we show that plant-compatible ABE systems can be successfully applied to protoplasts of Arabidopsis thaliana and Brassica napus through transient transfection, and to individual plants through Agrobacterium-mediated transformation to obtain organisms with desired phenotypes. Targeted, precise A-to-G substitutions generated a single amino acid change in the FT protein or mis-splicing of the PDS3 RNA transcript, and we could thereby obtain transgenic plants with late-flowering and albino phenotypes, respectively. Our results provide 'proof of concept' for in planta ABE applications that can lead to induced neo-functionalization or altered mRNA splicing, opening up new avenues for plant genome engineering and biotechnology.

Original language	English
Pages (from-to)	427-431
Number of pages	5
Journal	Nature Plants
Volume	4
Issue number	7
DOIs	https://doi.org/10.1038/s41477-018-0178-x
State	Published - 1 Jul 2018

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title = "Precision genome engineering through adenine base editing in plants",

abstract = "The recent development of adenine base editors (ABEs) has enabled efficient and precise A-to-G base conversions in higher eukaryotic cells. Here, we show that plant-compatible ABE systems can be successfully applied to protoplasts of Arabidopsis thaliana and Brassica napus through transient transfection, and to individual plants through Agrobacterium-mediated transformation to obtain organisms with desired phenotypes. Targeted, precise A-to-G substitutions generated a single amino acid change in the FT protein or mis-splicing of the PDS3 RNA transcript, and we could thereby obtain transgenic plants with late-flowering and albino phenotypes, respectively. Our results provide 'proof of concept' for in planta ABE applications that can lead to induced neo-functionalization or altered mRNA splicing, opening up new avenues for plant genome engineering and biotechnology.",

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AU - Kang, Beum Chang

AU - Yun, Jae Young

AU - Kim, Sang Tae

AU - Shin, You Jin

AU - Ryu, Jahee

AU - Choi, Minkyung

AU - Woo, Je Wook

AU - Kim, Jin Soo

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AB - The recent development of adenine base editors (ABEs) has enabled efficient and precise A-to-G base conversions in higher eukaryotic cells. Here, we show that plant-compatible ABE systems can be successfully applied to protoplasts of Arabidopsis thaliana and Brassica napus through transient transfection, and to individual plants through Agrobacterium-mediated transformation to obtain organisms with desired phenotypes. Targeted, precise A-to-G substitutions generated a single amino acid change in the FT protein or mis-splicing of the PDS3 RNA transcript, and we could thereby obtain transgenic plants with late-flowering and albino phenotypes, respectively. Our results provide 'proof of concept' for in planta ABE applications that can lead to induced neo-functionalization or altered mRNA splicing, opening up new avenues for plant genome engineering and biotechnology.

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Precision genome engineering through adenine base editing in plants

Abstract

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