Precision genome engineering through adenine base editing in plants

Beum Chang Kang; Jae Young Yun; Sang Tae Kim; You Jin Shin; Jahee Ryu; Minkyung Choi; Je Wook Woo; Jin Soo Kim

doi:10.1038/s41477-018-0178-x

Precision genome engineering through adenine base editing in plants

Beum Chang Kang, Jae Young Yun, Sang Tae Kim, You Jin Shin, Jahee Ryu, Minkyung Choi, Je Wook Woo, Jin Soo Kim

School of Convergence

Research output: Contribution to journal › Article › peer-review

263 Scopus citations

Abstract

The recent development of adenine base editors (ABEs) has enabled efficient and precise A-to-G base conversions in higher eukaryotic cells. Here, we show that plant-compatible ABE systems can be successfully applied to protoplasts of Arabidopsis thaliana and Brassica napus through transient transfection, and to individual plants through Agrobacterium-mediated transformation to obtain organisms with desired phenotypes. Targeted, precise A-to-G substitutions generated a single amino acid change in the FT protein or mis-splicing of the PDS3 RNA transcript, and we could thereby obtain transgenic plants with late-flowering and albino phenotypes, respectively. Our results provide 'proof of concept' for in planta ABE applications that can lead to induced neo-functionalization or altered mRNA splicing, opening up new avenues for plant genome engineering and biotechnology.

Original language	English
Pages (from-to)	427-431
Number of pages	5
Journal	Nature Plants
Volume	4
Issue number	7
DOIs	https://doi.org/10.1038/s41477-018-0178-x
State	Published - 1 Jul 2018

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abstract = "The recent development of adenine base editors (ABEs) has enabled efficient and precise A-to-G base conversions in higher eukaryotic cells. Here, we show that plant-compatible ABE systems can be successfully applied to protoplasts of Arabidopsis thaliana and Brassica napus through transient transfection, and to individual plants through Agrobacterium-mediated transformation to obtain organisms with desired phenotypes. Targeted, precise A-to-G substitutions generated a single amino acid change in the FT protein or mis-splicing of the PDS3 RNA transcript, and we could thereby obtain transgenic plants with late-flowering and albino phenotypes, respectively. Our results provide 'proof of concept' for in planta ABE applications that can lead to induced neo-functionalization or altered mRNA splicing, opening up new avenues for plant genome engineering and biotechnology.",

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AU - Kang, Beum Chang

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AU - Shin, You Jin

AU - Ryu, Jahee

AU - Choi, Minkyung

AU - Woo, Je Wook

AU - Kim, Jin Soo

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Precision genome engineering through adenine base editing in plants

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