Simple and rapid detection of bisphenol A using a gold nanoparticle-based colorimetric aptasensor

Eun Hee Lee; Sung Ku Lee; Min Jung Kim; Seung Woo Lee

doi:10.1016/j.foodchem.2019.02.079

Simple and rapid detection of bisphenol A using a gold nanoparticle-based colorimetric aptasensor

Eun Hee Lee
, Sung Ku Lee
, Min Jung Kim
, Seung Woo Lee

Dept. of Fine Chemistry

Research output: Contribution to journal › Article › peer-review

101 Scopus citations

Abstract

A colorimetric aptasensor was developed for the simple and rapid detection of bisphenol A (BPA). The aptasensor was designed to consist of colloidal gold nanoparticles (AuNPs) and a BPA-specific 24-bp aptamer. The AuNP-aptamer conjugates underwent an electrolyte-induced aggregation in the presence of sub-ppb levels of BPA. The surface plasmon resonance shift of AuNPs facilitated a color change from red to blue upon aggregation, which was visually observed by the naked eye. The corresponding visual limit of detection of BPA was as low as 1 pg/mL (0.004 nM). The aptasensor also achieved a selective detection of BPA over a variety of BPA analogs. The applicability of the aptasensor was verified via a successful detection of BPA in a single grain of rice. This result indicates that the colorimetric aptasensor can be used in a screening procedure for food and environmental monitoring, with reliable performance to sub-ppb levels of BPA detection.

Original language	English
Pages (from-to)	205-213
Number of pages	9
Journal	Food Chemistry
Volume	287
DOIs	https://doi.org/10.1016/j.foodchem.2019.02.079
State	Published - 30 Jul 2019

Keywords

Aptamer
Biosensor
Bisphenol A
Endocrine disruptor
Gold nanoparticles
Rice grain

Access to Document

10.1016/j.foodchem.2019.02.079

Cite this

@article{c8d024603b95467483ef78f720dcbace,

title = "Simple and rapid detection of bisphenol A using a gold nanoparticle-based colorimetric aptasensor",

abstract = "A colorimetric aptasensor was developed for the simple and rapid detection of bisphenol A (BPA). The aptasensor was designed to consist of colloidal gold nanoparticles (AuNPs) and a BPA-specific 24-bp aptamer. The AuNP-aptamer conjugates underwent an electrolyte-induced aggregation in the presence of sub-ppb levels of BPA. The surface plasmon resonance shift of AuNPs facilitated a color change from red to blue upon aggregation, which was visually observed by the naked eye. The corresponding visual limit of detection of BPA was as low as 1 pg/mL (0.004 nM). The aptasensor also achieved a selective detection of BPA over a variety of BPA analogs. The applicability of the aptasensor was verified via a successful detection of BPA in a single grain of rice. This result indicates that the colorimetric aptasensor can be used in a screening procedure for food and environmental monitoring, with reliable performance to sub-ppb levels of BPA detection.",

keywords = "Aptamer, Biosensor, Bisphenol A, Endocrine disruptor, Gold nanoparticles, Rice grain",

author = "Lee, \{Eun Hee\} and Lee, \{Sung Ku\} and Kim, \{Min Jung\} and Lee, \{Seung Woo\}",

note = "Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd",

year = "2019",

month = jul,

day = "30",

doi = "10.1016/j.foodchem.2019.02.079",

language = "English",

volume = "287",

pages = "205--213",

journal = "Food Chemistry",

issn = "0308-8146",

}

TY - JOUR

T1 - Simple and rapid detection of bisphenol A using a gold nanoparticle-based colorimetric aptasensor

AU - Lee, Eun Hee

AU - Lee, Sung Ku

AU - Kim, Min Jung

AU - Lee, Seung Woo

PY - 2019/7/30

Y1 - 2019/7/30

N2 - A colorimetric aptasensor was developed for the simple and rapid detection of bisphenol A (BPA). The aptasensor was designed to consist of colloidal gold nanoparticles (AuNPs) and a BPA-specific 24-bp aptamer. The AuNP-aptamer conjugates underwent an electrolyte-induced aggregation in the presence of sub-ppb levels of BPA. The surface plasmon resonance shift of AuNPs facilitated a color change from red to blue upon aggregation, which was visually observed by the naked eye. The corresponding visual limit of detection of BPA was as low as 1 pg/mL (0.004 nM). The aptasensor also achieved a selective detection of BPA over a variety of BPA analogs. The applicability of the aptasensor was verified via a successful detection of BPA in a single grain of rice. This result indicates that the colorimetric aptasensor can be used in a screening procedure for food and environmental monitoring, with reliable performance to sub-ppb levels of BPA detection.

AB - A colorimetric aptasensor was developed for the simple and rapid detection of bisphenol A (BPA). The aptasensor was designed to consist of colloidal gold nanoparticles (AuNPs) and a BPA-specific 24-bp aptamer. The AuNP-aptamer conjugates underwent an electrolyte-induced aggregation in the presence of sub-ppb levels of BPA. The surface plasmon resonance shift of AuNPs facilitated a color change from red to blue upon aggregation, which was visually observed by the naked eye. The corresponding visual limit of detection of BPA was as low as 1 pg/mL (0.004 nM). The aptasensor also achieved a selective detection of BPA over a variety of BPA analogs. The applicability of the aptasensor was verified via a successful detection of BPA in a single grain of rice. This result indicates that the colorimetric aptasensor can be used in a screening procedure for food and environmental monitoring, with reliable performance to sub-ppb levels of BPA detection.

KW - Aptamer

KW - Biosensor

KW - Bisphenol A

KW - Endocrine disruptor

KW - Gold nanoparticles

KW - Rice grain

UR - https://www.scopus.com/pages/publications/85062156781

U2 - 10.1016/j.foodchem.2019.02.079

DO - 10.1016/j.foodchem.2019.02.079

M3 - Article

C2 - 30857691

AN - SCOPUS:85062156781

SN - 0308-8146

VL - 287

SP - 205

EP - 213

JO - Food Chemistry

JF - Food Chemistry

ER -

Simple and rapid detection of bisphenol A using a gold nanoparticle-based colorimetric aptasensor

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this