Simple methods for tracking stem cells with 64Cu-labeled DOTA-hexadecyl-benzoate

Min Hwan Kim; Sang Keun Woo; Kwang Il Kim; Tae Sup Lee; Chan Wha Kim; Joo Hyun Kang; Byung Il Kim; Sang Moo Lim; Kyo Chul Lee; Yong Jin Lee

doi:10.1021/acsmedchemlett.5b00021

Simple methods for tracking stem cells with ⁶⁴Cu-labeled DOTA-hexadecyl-benzoate

Min Hwan Kim
, Sang Keun Woo
, Kwang Il Kim
, Tae Sup Lee
, Chan Wha Kim
, Joo Hyun Kang
, Byung Il Kim
, Sang Moo Lim
, Kyo Chul Lee
, Yong Jin Lee

Dept. of Biomedical Sciences

Korea Institute of Radiological and Medical Sciences
Korea University

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The purpose of this study was to evaluate ⁶⁴Cu-labeled hexadecyl-1,4,7,10-tetraazacyclododecane-tetraacetic acid-benzoate (⁶⁴Cu-DOTA-HB) (1) as positron emission tomography (PET) radiotracer for stem cell imaging. Hexadecyl-DOTA-benzoate (DOTA-HB) (2) was efficiently labeled with ⁶⁴Cu (>99%), and cell labeling efficiency with adipose-derived stem cells (ADSCs) was over 50%. Labeling with 1 did not compromise cell viability. In the PET imaging, intramuscularly transplanted 1-labeled ADSCs were monitored for 18 h in normal rat heart. These results indicate that 1 can be utilized as a promising radiotracer for monitoring of transplanted stem cells.

Original language	English
Pages (from-to)	528-530
Number of pages	3
Journal	ACS Medicinal Chemistry Letters
Volume	6
Issue number	5
DOIs	https://doi.org/10.1021/acsmedchemlett.5b00021
State	Published - 14 May 2015

Keywords

Adipose-derived stem cells
copper-64
hexadecyl benzoate
PET
radiotracer

Access to Document

10.1021/acsmedchemlett.5b00021

Cite this

@article{08febae36bb749e79f3ebfc96e2facc7,

title = "Simple methods for tracking stem cells with 64Cu-labeled DOTA-hexadecyl-benzoate",

abstract = "The purpose of this study was to evaluate 64Cu-labeled hexadecyl-1,4,7,10-tetraazacyclododecane-tetraacetic acid-benzoate (64Cu-DOTA-HB) (1) as positron emission tomography (PET) radiotracer for stem cell imaging. Hexadecyl-DOTA-benzoate (DOTA-HB) (2) was efficiently labeled with 64Cu (>99\%), and cell labeling efficiency with adipose-derived stem cells (ADSCs) was over 50\%. Labeling with 1 did not compromise cell viability. In the PET imaging, intramuscularly transplanted 1-labeled ADSCs were monitored for 18 h in normal rat heart. These results indicate that 1 can be utilized as a promising radiotracer for monitoring of transplanted stem cells.",

keywords = "Adipose-derived stem cells, copper-64, hexadecyl benzoate, PET, radiotracer",

author = "Kim, \{Min Hwan\} and Woo, \{Sang Keun\} and Kim, \{Kwang Il\} and Lee, \{Tae Sup\} and Kim, \{Chan Wha\} and Kang, \{Joo Hyun\} and Kim, \{Byung Il\} and Lim, \{Sang Moo\} and Lee, \{Kyo Chul\} and Lee, \{Yong Jin\}",

note = "Publisher Copyright: {\textcopyright} 2015 American Chemical Society.",

year = "2015",

month = may,

day = "14",

doi = "10.1021/acsmedchemlett.5b00021",

language = "English",

volume = "6",

pages = "528--530",

journal = "ACS Medicinal Chemistry Letters",

issn = "1948-5875",

number = "5",

}

TY - JOUR

T1 - Simple methods for tracking stem cells with 64Cu-labeled DOTA-hexadecyl-benzoate

AU - Kim, Min Hwan

AU - Woo, Sang Keun

AU - Kim, Kwang Il

AU - Lee, Tae Sup

AU - Kim, Chan Wha

AU - Kang, Joo Hyun

AU - Kim, Byung Il

AU - Lim, Sang Moo

AU - Lee, Kyo Chul

AU - Lee, Yong Jin

PY - 2015/5/14

Y1 - 2015/5/14

N2 - The purpose of this study was to evaluate 64Cu-labeled hexadecyl-1,4,7,10-tetraazacyclododecane-tetraacetic acid-benzoate (64Cu-DOTA-HB) (1) as positron emission tomography (PET) radiotracer for stem cell imaging. Hexadecyl-DOTA-benzoate (DOTA-HB) (2) was efficiently labeled with 64Cu (>99%), and cell labeling efficiency with adipose-derived stem cells (ADSCs) was over 50%. Labeling with 1 did not compromise cell viability. In the PET imaging, intramuscularly transplanted 1-labeled ADSCs were monitored for 18 h in normal rat heart. These results indicate that 1 can be utilized as a promising radiotracer for monitoring of transplanted stem cells.

AB - The purpose of this study was to evaluate 64Cu-labeled hexadecyl-1,4,7,10-tetraazacyclododecane-tetraacetic acid-benzoate (64Cu-DOTA-HB) (1) as positron emission tomography (PET) radiotracer for stem cell imaging. Hexadecyl-DOTA-benzoate (DOTA-HB) (2) was efficiently labeled with 64Cu (>99%), and cell labeling efficiency with adipose-derived stem cells (ADSCs) was over 50%. Labeling with 1 did not compromise cell viability. In the PET imaging, intramuscularly transplanted 1-labeled ADSCs were monitored for 18 h in normal rat heart. These results indicate that 1 can be utilized as a promising radiotracer for monitoring of transplanted stem cells.

KW - Adipose-derived stem cells

KW - copper-64

KW - hexadecyl benzoate

KW - PET

KW - radiotracer

UR - https://www.scopus.com/pages/publications/84929336431

U2 - 10.1021/acsmedchemlett.5b00021

DO - 10.1021/acsmedchemlett.5b00021

M3 - Article

AN - SCOPUS:84929336431

SN - 1948-5875

VL - 6

SP - 528

EP - 530

JO - ACS Medicinal Chemistry Letters

JF - ACS Medicinal Chemistry Letters

IS - 5

ER -

Simple methods for tracking stem cells with ⁶⁴Cu-labeled DOTA-hexadecyl-benzoate

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this