Urea Conversion via Enzymes Immobilized on Magnetic Microparticles

Yeseul Park; Jieun Kwon; Seungjun Jung; Hyukjin Cho; Gounhanul Shin; Gangik Cho; Jin Won Park; Kyung Hyuk Lee

doi:10.9713/kcer.2023.61.3.407

Urea Conversion via Enzymes Immobilized on Magnetic Microparticles

Yeseul Park, Jieun Kwon, Seungjun Jung, Hyukjin Cho, Gounhanul Shin, Gangik Cho, Jin Won Park, Kyung Hyuk Lee

Dept. of Chemical and Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

The urease is covalently immobilized on the surface of the magnetic particles to catalyze the conversion of urea to bicarbonate anion. The conversion was confirmed qualitatively using high-pressure liquid chromatography and UV/Vis spectrometry, and analyzed quantitatively with cyclic voltammetry. The amount of conversion with respect to time was measured and analyzed by the reaction rate equation to calculate a reaction rate constant of 0.0474 min^-1. In the 1 to 3 cycles, a conversion percentage of over 90% was found, and it was possible to reuse the urease 8 times up to the percentage of 50%. It was also observed that the stability evaluated for storage for 30 days was maintained. As a result of this study, it can be seen that the urease covalently immobilized on the scaffold can be used for urea removal for the purpose of producing ultrapure water.

Original language	English
Pages (from-to)	407-411
Number of pages	5
Journal	Korean Chemical Engineering Research
Volume	61
Issue number	3
DOIs	https://doi.org/10.9713/kcer.2023.61.3.407
State	Published - Aug 2023

Keywords

Bicarbonate
Enzyme immobilization
Magnetic particles
Urea

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10.9713/kcer.2023.61.3.407

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title = "Urea Conversion via Enzymes Immobilized on Magnetic Microparticles",

abstract = "The urease is covalently immobilized on the surface of the magnetic particles to catalyze the conversion of urea to bicarbonate anion. The conversion was confirmed qualitatively using high-pressure liquid chromatography and UV/Vis spectrometry, and analyzed quantitatively with cyclic voltammetry. The amount of conversion with respect to time was measured and analyzed by the reaction rate equation to calculate a reaction rate constant of 0.0474 min-1. In the 1 to 3 cycles, a conversion percentage of over 90\% was found, and it was possible to reuse the urease 8 times up to the percentage of 50\%. It was also observed that the stability evaluated for storage for 30 days was maintained. As a result of this study, it can be seen that the urease covalently immobilized on the scaffold can be used for urea removal for the purpose of producing ultrapure water.",

keywords = "Bicarbonate, Enzyme immobilization, Magnetic particles, Urea",

author = "Yeseul Park and Jieun Kwon and Seungjun Jung and Hyukjin Cho and Gounhanul Shin and Gangik Cho and Park, \{Jin Won\} and Lee, \{Kyung Hyuk\}",

year = "2023",

month = aug,

doi = "10.9713/kcer.2023.61.3.407",

language = "English",

volume = "61",

pages = "407--411",

journal = "Korean Chemical Engineering Research",

issn = "0304-128X",

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TY - JOUR

T1 - Urea Conversion via Enzymes Immobilized on Magnetic Microparticles

AU - Park, Yeseul

AU - Kwon, Jieun

AU - Jung, Seungjun

AU - Cho, Hyukjin

AU - Shin, Gounhanul

AU - Cho, Gangik

AU - Park, Jin Won

AU - Lee, Kyung Hyuk

PY - 2023/8

Y1 - 2023/8

N2 - The urease is covalently immobilized on the surface of the magnetic particles to catalyze the conversion of urea to bicarbonate anion. The conversion was confirmed qualitatively using high-pressure liquid chromatography and UV/Vis spectrometry, and analyzed quantitatively with cyclic voltammetry. The amount of conversion with respect to time was measured and analyzed by the reaction rate equation to calculate a reaction rate constant of 0.0474 min-1. In the 1 to 3 cycles, a conversion percentage of over 90% was found, and it was possible to reuse the urease 8 times up to the percentage of 50%. It was also observed that the stability evaluated for storage for 30 days was maintained. As a result of this study, it can be seen that the urease covalently immobilized on the scaffold can be used for urea removal for the purpose of producing ultrapure water.

AB - The urease is covalently immobilized on the surface of the magnetic particles to catalyze the conversion of urea to bicarbonate anion. The conversion was confirmed qualitatively using high-pressure liquid chromatography and UV/Vis spectrometry, and analyzed quantitatively with cyclic voltammetry. The amount of conversion with respect to time was measured and analyzed by the reaction rate equation to calculate a reaction rate constant of 0.0474 min-1. In the 1 to 3 cycles, a conversion percentage of over 90% was found, and it was possible to reuse the urease 8 times up to the percentage of 50%. It was also observed that the stability evaluated for storage for 30 days was maintained. As a result of this study, it can be seen that the urease covalently immobilized on the scaffold can be used for urea removal for the purpose of producing ultrapure water.

KW - Bicarbonate

KW - Enzyme immobilization

KW - Magnetic particles

KW - Urea

UR - https://www.scopus.com/pages/publications/85171660109

U2 - 10.9713/kcer.2023.61.3.407

DO - 10.9713/kcer.2023.61.3.407

M3 - Article

AN - SCOPUS:85171660109

SN - 0304-128X

VL - 61

SP - 407

EP - 411

JO - Korean Chemical Engineering Research

JF - Korean Chemical Engineering Research

IS - 3

ER -

Urea Conversion via Enzymes Immobilized on Magnetic Microparticles

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