Abstract
Purpose: The present study was conducted to investigate whether the changes in structure and activity ofantioxidative enzymes, superoxide dismutase(SOD) and catalase(CAT) present in the eyes appeared when theywere repeatedly exposed to UV-A, and reveal the correlation of these changes. Methods: Each enzyme solutionwas prepared from the standardized SOD and CAT, and repeatedly exposed to UV-A of 365 min under thecondition of 30 minutes, 1 hour and 2 hours a day over 1, 2, 3, 4 and 5 days. Structural denaturation of SOD andCAT induced by repeat UV-A irradiation was confirmed by the electrophoretic analysis, and their enzyme activitywas determined by the colorimetric assay using the proper assay kit. Results: SOD exposed repeatedly to UV-Ashowed the polymerization pattern through the electrophoretic analysis when it was repeatedly exposed under thecondition of at least 1 hour a day however, the change of its activity was found to be less than 12%. On the otherhand, CAT repeatedly exposed to UV-A showed reduced size of the electrophoretic band which indicated astructure denaturation and its activity was significantly decreased. In the case of that the repeat exposure time waslonger, CAT activity was completely lost even though some enzyme band was shown in the electrphoreticanalysis. Conclusions: From these results, it was revealed that the degree and pattern in structural denaturation ofantioxidative enzymes differently appeared according to the type of enzyme, and the degree of structuraldenaturation was not always consistent with the reduction in enzyme activity.
| Translated title of the contribution | The Relationship between Structural Denaturation of Antioxidative Enzymes and Their Enzyme Activity due to Repeated Exposure to UV-A |
|---|---|
| Original language | Korean |
| Pages (from-to) | 75-81 |
| Number of pages | 7 |
| Journal | 한국안광학회지 |
| Volume | 20 |
| Issue number | 1 |
| DOIs | |
| State | Published - Mar 2015 |